miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2537 3' -59.4 NC_001460.1 + 15293 1.13 9.9e-05
Target:  5'- gGGCUGCAACGCCUGCCGGUCGAGCGCg -3'
miRNA:   3'- -CCGACGUUGCGGACGGCCAGCUCGCG- -5'
2537 3' -59.4 NC_001460.1 + 10241 0.74 0.109199
Target:  5'- -aCUGCGcACGCCUGaCCGGUCaaAGCGUg -3'
miRNA:   3'- ccGACGU-UGCGGAC-GGCCAGc-UCGCG- -5'
2537 3' -59.4 NC_001460.1 + 5902 0.7 0.217494
Target:  5'- ---gGUAAUGCCUGCCu-UUGAGCGCg -3'
miRNA:   3'- ccgaCGUUGCGGACGGccAGCUCGCG- -5'
2537 3' -59.4 NC_001460.1 + 10174 0.68 0.274932
Target:  5'- aGUUGCcACGCuUUGaCCGGUCaGGCGUg -3'
miRNA:   3'- cCGACGuUGCG-GAC-GGCCAGcUCGCG- -5'
2537 3' -59.4 NC_001460.1 + 22582 0.67 0.327547
Target:  5'- uGGCgGCGGCGgCUGagcgcugCGGUCugGGGUGCg -3'
miRNA:   3'- -CCGaCGUUGCgGACg------GCCAG--CUCGCG- -5'
2537 3' -59.4 NC_001460.1 + 15164 0.66 0.352276
Target:  5'- gGGCuuaUGCAagGCGCCgcagacgGCUGcGUCGccGGCGUa -3'
miRNA:   3'- -CCG---ACGU--UGCGGa------CGGC-CAGC--UCGCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.