Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2537 | 3' | -59.4 | NC_001460.1 | + | 15293 | 1.13 | 9.9e-05 |
Target: 5'- gGGCUGCAACGCCUGCCGGUCGAGCGCg -3' miRNA: 3'- -CCGACGUUGCGGACGGCCAGCUCGCG- -5' |
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2537 | 3' | -59.4 | NC_001460.1 | + | 10241 | 0.74 | 0.109199 |
Target: 5'- -aCUGCGcACGCCUGaCCGGUCaaAGCGUg -3' miRNA: 3'- ccGACGU-UGCGGAC-GGCCAGc-UCGCG- -5' |
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2537 | 3' | -59.4 | NC_001460.1 | + | 5902 | 0.7 | 0.217494 |
Target: 5'- ---gGUAAUGCCUGCCu-UUGAGCGCg -3' miRNA: 3'- ccgaCGUUGCGGACGGccAGCUCGCG- -5' |
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2537 | 3' | -59.4 | NC_001460.1 | + | 10174 | 0.68 | 0.274932 |
Target: 5'- aGUUGCcACGCuUUGaCCGGUCaGGCGUg -3' miRNA: 3'- cCGACGuUGCG-GAC-GGCCAGcUCGCG- -5' |
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2537 | 3' | -59.4 | NC_001460.1 | + | 22582 | 0.67 | 0.327547 |
Target: 5'- uGGCgGCGGCGgCUGagcgcugCGGUCugGGGUGCg -3' miRNA: 3'- -CCGaCGUUGCgGACg------GCCAG--CUCGCG- -5' |
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2537 | 3' | -59.4 | NC_001460.1 | + | 15164 | 0.66 | 0.352276 |
Target: 5'- gGGCuuaUGCAagGCGCCgcagacgGCUGcGUCGccGGCGUa -3' miRNA: 3'- -CCG---ACGU--UGCGGa------CGGC-CAGC--UCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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