Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25386 | 3' | -58.5 | NC_005336.1 | + | 64697 | 0.66 | 0.755839 |
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Target: 5'- -cCUUCCUGgacuucgaguUCGCCGGCgGAGAc -3' miRNA: 3'- caGAAGGACaac-------GGCGGCCGgCUCUa -5' |
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| 25386 | 3' | -58.5 | NC_005336.1 | + | 71415 | 0.66 | 0.748131 |
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Target: 5'- -cCUUCCac-UGCCGCgUGGCCGAGc- -3' miRNA: 3'- caGAAGGacaACGGCG-GCCGGCUCua -5' |
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| 25386 | 3' | -58.5 | NC_005336.1 | + | 41878 | 0.66 | 0.748131 |
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Target: 5'- cGUCUUCCUGUucuUGgCGUCa-CCGAGAa -3' miRNA: 3'- -CAGAAGGACA---ACgGCGGccGGCUCUa -5' |
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| 25386 | 3' | -58.5 | NC_005336.1 | + | 94184 | 0.67 | 0.728587 |
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Target: 5'- gGUCggcgCCggugcgggGUUGUCGCCGGacgaCGAGGUa -3' miRNA: 3'- -CAGaa--GGa-------CAACGGCGGCCg---GCUCUA- -5' |
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| 25386 | 3' | -58.5 | NC_005336.1 | + | 133879 | 0.67 | 0.678425 |
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Target: 5'- -cCUUCCUGgaG-CGCgCGGCCGAGu- -3' miRNA: 3'- caGAAGGACaaCgGCG-GCCGGCUCua -5' |
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| 25386 | 3' | -58.5 | NC_005336.1 | + | 87781 | 0.67 | 0.676392 |
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Target: 5'- ---gUCCUGgacaccgcaggGCCGCCGGUCGaAGAg -3' miRNA: 3'- cagaAGGACaa---------CGGCGGCCGGC-UCUa -5' |
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| 25386 | 3' | -58.5 | NC_005336.1 | + | 93807 | 0.68 | 0.658031 |
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Target: 5'- ---cUCCUGUgcUGCUGCUGGaCCGAGu- -3' miRNA: 3'- cagaAGGACA--ACGGCGGCC-GGCUCua -5' |
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| 25386 | 3' | -58.5 | NC_005336.1 | + | 96777 | 0.69 | 0.606824 |
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Target: 5'- cUCggcgUCCUGaacGCCGCCGGCgGuGAa -3' miRNA: 3'- cAGa---AGGACaa-CGGCGGCCGgCuCUa -5' |
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| 25386 | 3' | -58.5 | NC_005336.1 | + | 65676 | 1.06 | 0.002088 |
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Target: 5'- cGUCUUCCUGUUGCCGCCGGCCGAGAUg -3' miRNA: 3'- -CAGAAGGACAACGGCGGCCGGCUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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