Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25503 | 3' | -56.6 | NC_005337.1 | + | 45544 | 0.68 | 0.763073 |
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Target: 5'- cGGUgCUgGAGUCGGCgCCG-CCGUGg- -3' miRNA: 3'- -UCAaGAgCUCAGUCG-GGCaGGCACgc -5' |
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| 25503 | 3' | -56.6 | NC_005337.1 | + | 112080 | 0.68 | 0.781772 |
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Target: 5'- --cUCUCG-GUgAGCCCG-CUGUGCc -3' miRNA: 3'- ucaAGAGCuCAgUCGGGCaGGCACGc -5' |
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| 25503 | 3' | -56.6 | NC_005337.1 | + | 90102 | 0.68 | 0.799936 |
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Target: 5'- --cUCUCGccGGUCAGCUgGUCgGUGuCGa -3' miRNA: 3'- ucaAGAGC--UCAGUCGGgCAGgCAC-GC- -5' |
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| 25503 | 3' | -56.6 | NC_005337.1 | + | 128032 | 0.67 | 0.808795 |
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Target: 5'- -----aCGAGUCGGCCaaggCCGUGCa -3' miRNA: 3'- ucaagaGCUCAGUCGGgca-GGCACGc -5' |
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| 25503 | 3' | -56.6 | NC_005337.1 | + | 12251 | 0.67 | 0.817496 |
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Target: 5'- uGUUCUCGAG-CAuGUCCGUCgCG-GCc -3' miRNA: 3'- uCAAGAGCUCaGU-CGGGCAG-GCaCGc -5' |
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| 25503 | 3' | -56.6 | NC_005337.1 | + | 90282 | 0.67 | 0.834385 |
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Target: 5'- gAGUUCUCGAGcgucUCGGCCUccucgccgCCGgGCGc -3' miRNA: 3'- -UCAAGAGCUC----AGUCGGGca------GGCaCGC- -5' |
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| 25503 | 3' | -56.6 | NC_005337.1 | + | 100691 | 0.67 | 0.842559 |
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Target: 5'- cGggCUCGAGaCGGUCUGUCuCG-GCGa -3' miRNA: 3'- uCaaGAGCUCaGUCGGGCAG-GCaCGC- -5' |
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| 25503 | 3' | -56.6 | NC_005337.1 | + | 13989 | 0.66 | 0.880443 |
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Target: 5'- cGUgCUCGAGUUAGUCCGgaucUUCGUGa- -3' miRNA: 3'- uCAaGAGCUCAGUCGGGC----AGGCACgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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