Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25506 | 5' | -50.5 | NC_005337.1 | + | 53411 | 0.66 | 0.991846 |
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Target: 5'- -aUCGAGAUCUUCAaGCAgcuggucuucuUCUGCCa-- -3' miRNA: 3'- cgAGUUCUAGGAGU-CGU-----------AGAUGGcac -5' |
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| 25506 | 5' | -50.5 | NC_005337.1 | + | 82274 | 0.68 | 0.975192 |
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Target: 5'- cGUUCGAGGUCgUgAGCAUCUuCC-UGg -3' miRNA: 3'- -CGAGUUCUAGgAgUCGUAGAuGGcAC- -5' |
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| 25506 | 5' | -50.5 | NC_005337.1 | + | 122638 | 0.68 | 0.972404 |
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Target: 5'- cGCcCGAGAaguUCCUgGGCuUCUACCGc- -3' miRNA: 3'- -CGaGUUCU---AGGAgUCGuAGAUGGCac -5' |
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| 25506 | 5' | -50.5 | NC_005337.1 | + | 85415 | 0.68 | 0.969396 |
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Target: 5'- cGC-CGAGAUCagCAGCAUCUcCCGg- -3' miRNA: 3'- -CGaGUUCUAGgaGUCGUAGAuGGCac -5' |
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| 25506 | 5' | -50.5 | NC_005337.1 | + | 95118 | 0.68 | 0.969396 |
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Target: 5'- cGCUCGcGGucGUCCUcCAGCGUC-GCCGUc -3' miRNA: 3'- -CGAGU-UC--UAGGA-GUCGUAGaUGGCAc -5' |
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| 25506 | 5' | -50.5 | NC_005337.1 | + | 38113 | 0.68 | 0.966162 |
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Target: 5'- uGCUCGGGcUCCUCuucGCcAUCUACgCGUu -3' miRNA: 3'- -CGAGUUCuAGGAGu--CG-UAGAUG-GCAc -5' |
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| 25506 | 5' | -50.5 | NC_005337.1 | + | 87459 | 0.69 | 0.94637 |
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Target: 5'- cGCUgGAGAUggccagcgcgcCCUCGGCGUCccagACCGa- -3' miRNA: 3'- -CGAgUUCUA-----------GGAGUCGUAGa---UGGCac -5' |
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| 25506 | 5' | -50.5 | NC_005337.1 | + | 80841 | 0.71 | 0.887406 |
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Target: 5'- aGCUCucGAUCUUguGCGUC-ACCGUc -3' miRNA: 3'- -CGAGuuCUAGGAguCGUAGaUGGCAc -5' |
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| 25506 | 5' | -50.5 | NC_005337.1 | + | 45519 | 0.77 | 0.607587 |
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Target: 5'- cCUCcGGGUCCUCGGCGUCgggaGCCGg- -3' miRNA: 3'- cGAGuUCUAGGAGUCGUAGa---UGGCac -5' |
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| 25506 | 5' | -50.5 | NC_005337.1 | + | 131440 | 1.12 | 0.005125 |
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Target: 5'- cGCUCAAGAUCCUCAGCAUCUACCGUGa -3' miRNA: 3'- -CGAGUUCUAGGAGUCGUAGAUGGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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