Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25509 | 3' | -57.3 | NC_005337.1 | + | 66917 | 0.66 | 0.861032 |
Target: 5'- aUGUACUcggUCaugGGCUGcGCCaUCGUGGAg -3' miRNA: 3'- gACAUGG---AGg--CUGAC-CGGcAGCACCU- -5' |
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25509 | 3' | -57.3 | NC_005337.1 | + | 115550 | 0.66 | 0.836616 |
Target: 5'- gUGUucuCCUCCGAgcacagcgcgcgaCUGGCCG-CGUGu- -3' miRNA: 3'- gACAu--GGAGGCU-------------GACCGGCaGCACcu -5' |
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25509 | 3' | -57.3 | NC_005337.1 | + | 30039 | 0.67 | 0.812181 |
Target: 5'- -cGUGCCcuUCUGGCUGGUgCG-CGUGGu -3' miRNA: 3'- gaCAUGG--AGGCUGACCG-GCaGCACCu -5' |
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25509 | 3' | -57.3 | NC_005337.1 | + | 52114 | 0.67 | 0.803432 |
Target: 5'- -gGUGCgCgacgggCCGGCcaUGGCCuUCGUGGAc -3' miRNA: 3'- gaCAUG-Ga-----GGCUG--ACCGGcAGCACCU- -5' |
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25509 | 3' | -57.3 | NC_005337.1 | + | 55007 | 0.67 | 0.802548 |
Target: 5'- -gGUACCagcagUCUGagggcgacuucguGCUGGCCuUCGUGGAg -3' miRNA: 3'- gaCAUGG-----AGGC-------------UGACCGGcAGCACCU- -5' |
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25509 | 3' | -57.3 | NC_005337.1 | + | 129358 | 0.67 | 0.794529 |
Target: 5'- -cGUG-CUCCGGgaGGCCGUCGUc-- -3' miRNA: 3'- gaCAUgGAGGCUgaCCGGCAGCAccu -5' |
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25509 | 3' | -57.3 | NC_005337.1 | + | 75608 | 0.67 | 0.794529 |
Target: 5'- gUGUACCUggggagcgCCGaggacGCgcgGGCCGUCGUGa- -3' miRNA: 3'- gACAUGGA--------GGC-----UGa--CCGGCAGCACcu -5' |
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25509 | 3' | -57.3 | NC_005337.1 | + | 48307 | 0.67 | 0.776301 |
Target: 5'- -aGUACCUCCaGCggGGCaCGgggaugugCGUGGAc -3' miRNA: 3'- gaCAUGGAGGcUGa-CCG-GCa-------GCACCU- -5' |
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25509 | 3' | -57.3 | NC_005337.1 | + | 119547 | 0.68 | 0.718854 |
Target: 5'- -gGUGCag-CGGCUGGCgaugggCGUCGUGGAg -3' miRNA: 3'- gaCAUGgagGCUGACCG------GCAGCACCU- -5' |
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25509 | 3' | -57.3 | NC_005337.1 | + | 29363 | 0.69 | 0.699024 |
Target: 5'- -gGUGCCcUCGACggcGGCCGUCGcGGc -3' miRNA: 3'- gaCAUGGaGGCUGa--CCGGCAGCaCCu -5' |
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25509 | 3' | -57.3 | NC_005337.1 | + | 80102 | 0.7 | 0.638483 |
Target: 5'- -cGUGCCUgaagCCGAgcCaGGCgGUCGUGGAc -3' miRNA: 3'- gaCAUGGA----GGCU--GaCCGgCAGCACCU- -5' |
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25509 | 3' | -57.3 | NC_005337.1 | + | 75099 | 0.71 | 0.597911 |
Target: 5'- -gGUGCC-CCGACgcacGGCCugggggaggaacGUCGUGGGg -3' miRNA: 3'- gaCAUGGaGGCUGa---CCGG------------CAGCACCU- -5' |
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25509 | 3' | -57.3 | NC_005337.1 | + | 33404 | 0.74 | 0.399663 |
Target: 5'- -aGUAgCUCUGGC-GGCCGcCGUGGAu -3' miRNA: 3'- gaCAUgGAGGCUGaCCGGCaGCACCU- -5' |
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25509 | 3' | -57.3 | NC_005337.1 | + | 130365 | 1.08 | 0.002278 |
Target: 5'- cCUGUACCUCCGACUGGCCGUCGUGGAc -3' miRNA: 3'- -GACAUGGAGGCUGACCGGCAGCACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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