Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25513 | 3' | -48.1 | NC_005337.1 | + | 68245 | 0.66 | 0.997701 |
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Target: 5'- ---gGAGCGCGGCCGGcgcgUGG-GCGAGc -3' miRNA: 3'- augaCUCGCGUUGGUUa---AUCaCGUUU- -5' |
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| 25513 | 3' | -48.1 | NC_005337.1 | + | 102236 | 0.66 | 0.997701 |
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Target: 5'- gGCUGuGGCGCGugCAGacggccgGGUGCGu- -3' miRNA: 3'- aUGAC-UCGCGUugGUUaa-----UCACGUuu -5' |
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| 25513 | 3' | -48.1 | NC_005337.1 | + | 128642 | 0.67 | 0.993513 |
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Target: 5'- cUGCUGGauauccuGCGCGGCCGGUc-GUGCAc- -3' miRNA: 3'- -AUGACU-------CGCGUUGGUUAauCACGUuu -5' |
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| 25513 | 3' | -48.1 | NC_005337.1 | + | 87616 | 0.68 | 0.983034 |
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Target: 5'- cUGCUGcgcgaucgcGCGCAGCCccgacucgcggaGGUUGGUGCGGAa -3' miRNA: 3'- -AUGACu--------CGCGUUGG------------UUAAUCACGUUU- -5' |
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| 25513 | 3' | -48.1 | NC_005337.1 | + | 3375 | 0.7 | 0.966324 |
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Target: 5'- aGgUGuGCGCGGCCAugaGGUGCAGc -3' miRNA: 3'- aUgACuCGCGUUGGUuaaUCACGUUu -5' |
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| 25513 | 3' | -48.1 | NC_005337.1 | + | 98669 | 0.7 | 0.950175 |
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Target: 5'- gUGCUGGGCGgAGCCAc--GGUGCu-- -3' miRNA: 3'- -AUGACUCGCgUUGGUuaaUCACGuuu -5' |
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| 25513 | 3' | -48.1 | NC_005337.1 | + | 99670 | 0.71 | 0.935135 |
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Target: 5'- gGCUGGGCGCGcCCGuggcGUGCGAc -3' miRNA: 3'- aUGACUCGCGUuGGUuaauCACGUUu -5' |
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| 25513 | 3' | -48.1 | NC_005337.1 | + | 89305 | 0.72 | 0.897245 |
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Target: 5'- aGCguguugGAGCGCAGCCGGUgcucgcagaAGUGCAu- -3' miRNA: 3'- aUGa-----CUCGCGUUGGUUAa--------UCACGUuu -5' |
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| 25513 | 3' | -48.1 | NC_005337.1 | + | 35757 | 0.75 | 0.76211 |
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Target: 5'- cGCUGGGCGCGACCGGgaucgUGGcGCGu- -3' miRNA: 3'- aUGACUCGCGUUGGUUa----AUCaCGUuu -5' |
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| 25513 | 3' | -48.1 | NC_005337.1 | + | 126503 | 1 | 0.040017 |
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Target: 5'- cAUUGAGCGCAACCAAUUAGUGCAAAg -3' miRNA: 3'- aUGACUCGCGUUGGUUAAUCACGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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