Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25515 | 5' | -52.9 | NC_005337.1 | + | 60279 | 0.66 | 0.969254 |
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Target: 5'- -aGGcgCGGGGGUGgGA-GUGggCUUg -3' miRNA: 3'- gaCCuaGUCCCCAUgCUgCACaaGAG- -5' |
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| 25515 | 5' | -52.9 | NC_005337.1 | + | 36705 | 0.66 | 0.962696 |
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Target: 5'- -cGGAguUCAGGGGcuUGugGaagagGUUCUCg -3' miRNA: 3'- gaCCU--AGUCCCCauGCugCa----CAAGAG- -5' |
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| 25515 | 5' | -52.9 | NC_005337.1 | + | 12229 | 0.67 | 0.942214 |
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Target: 5'- gUGGc-CAGGcuGUgcaGCGGCGUGUUCUCg -3' miRNA: 3'- gACCuaGUCCc-CA---UGCUGCACAAGAG- -5' |
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| 25515 | 5' | -52.9 | NC_005337.1 | + | 10731 | 0.69 | 0.896547 |
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Target: 5'- aUGuGGUCAGGgcGGUGgGACGUGcaCUCg -3' miRNA: 3'- gAC-CUAGUCC--CCAUgCUGCACaaGAG- -5' |
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| 25515 | 5' | -52.9 | NC_005337.1 | + | 115525 | 0.69 | 0.889723 |
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Target: 5'- cCUGGAggAGGGcUACGAgcaggaaGUGUUCUCc -3' miRNA: 3'- -GACCUagUCCCcAUGCUg------CACAAGAG- -5' |
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| 25515 | 5' | -52.9 | NC_005337.1 | + | 37360 | 0.69 | 0.875363 |
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Target: 5'- uCUGGuu--GGGGUACagcgagggcaGCGUGUUCUCg -3' miRNA: 3'- -GACCuaguCCCCAUGc---------UGCACAAGAG- -5' |
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| 25515 | 5' | -52.9 | NC_005337.1 | + | 122600 | 0.73 | 0.701876 |
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Target: 5'- cCUGGAcCAcGcGGGcgGCGGCGUGUUCUUc -3' miRNA: 3'- -GACCUaGU-C-CCCa-UGCUGCACAAGAG- -5' |
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| 25515 | 5' | -52.9 | NC_005337.1 | + | 16573 | 0.74 | 0.649777 |
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Target: 5'- cCUGGAUCAGuGGGUgAUGACGU-UUCUg -3' miRNA: 3'- -GACCUAGUC-CCCA-UGCUGCAcAAGAg -5' |
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| 25515 | 5' | -52.9 | NC_005337.1 | + | 45345 | 0.74 | 0.649777 |
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Target: 5'- -cGGAUCAuGGGGcGCGAgGUGgugCUCa -3' miRNA: 3'- gaCCUAGU-CCCCaUGCUgCACaa-GAG- -5' |
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| 25515 | 5' | -52.9 | NC_005337.1 | + | 125885 | 1.11 | 0.00388 |
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Target: 5'- cCUGGAUCAGGGGUACGACGUGUUCUCa -3' miRNA: 3'- -GACCUAGUCCCCAUGCUGCACAAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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