Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25518 | 3' | -52.6 | NC_005337.1 | + | 39667 | 0.66 | 0.961907 |
Target: 5'- gGGGCGCGUGGCCccgggcacGUACGugcGCUUCUu -3' miRNA: 3'- -CUCGUGUACUGGca------CAUGC---CGAAGAu -5' |
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25518 | 3' | -52.6 | NC_005337.1 | + | 130786 | 0.66 | 0.95816 |
Target: 5'- uGGGCGCc--GCCG-GUGCGGCUgCUGc -3' miRNA: 3'- -CUCGUGuacUGGCaCAUGCCGAaGAU- -5' |
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25518 | 3' | -52.6 | NC_005337.1 | + | 131398 | 0.66 | 0.95816 |
Target: 5'- gGGGC-CGUGAUCGUGUucccgcuggcgcGCGGCUcCg- -3' miRNA: 3'- -CUCGuGUACUGGCACA------------UGCCGAaGau -5' |
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25518 | 3' | -52.6 | NC_005337.1 | + | 54249 | 0.67 | 0.940681 |
Target: 5'- cGGCGCGUccgGGCCGgGUGCGGCg---- -3' miRNA: 3'- cUCGUGUA---CUGGCaCAUGCCGaagau -5' |
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25518 | 3' | -52.6 | NC_005337.1 | + | 43701 | 0.67 | 0.940681 |
Target: 5'- cGGGUccACGUGAgcucguCCGUGUACGGCa---- -3' miRNA: 3'- -CUCG--UGUACU------GGCACAUGCCGaagau -5' |
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25518 | 3' | -52.6 | NC_005337.1 | + | 111147 | 0.67 | 0.924863 |
Target: 5'- gGGGCGCAUGGCCGcGaccACGGCg---- -3' miRNA: 3'- -CUCGUGUACUGGCaCa--UGCCGaagau -5' |
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25518 | 3' | -52.6 | NC_005337.1 | + | 48801 | 0.68 | 0.919067 |
Target: 5'- aAGCugGUGGCCGUGUucgACaGCUcgCUGc -3' miRNA: 3'- cUCGugUACUGGCACA---UGcCGAa-GAU- -5' |
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25518 | 3' | -52.6 | NC_005337.1 | + | 99977 | 0.68 | 0.906692 |
Target: 5'- uAGCgGCAUGAUCGUG-GCGGcCUUCg- -3' miRNA: 3'- cUCG-UGUACUGGCACaUGCC-GAAGau -5' |
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25518 | 3' | -52.6 | NC_005337.1 | + | 12890 | 0.7 | 0.830087 |
Target: 5'- cGGGCAaCAUGugCuUGUGCGGCaUCUu -3' miRNA: 3'- -CUCGU-GUACugGcACAUGCCGaAGAu -5' |
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25518 | 3' | -52.6 | NC_005337.1 | + | 51235 | 0.7 | 0.821217 |
Target: 5'- cAGCAgCAUGcugacgguguccGCCGUGUACGGCgUCa- -3' miRNA: 3'- cUCGU-GUAC------------UGGCACAUGCCGaAGau -5' |
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25518 | 3' | -52.6 | NC_005337.1 | + | 92191 | 0.77 | 0.45297 |
Target: 5'- cGAGCGCGUGACCGUGgcgcACGGUa---- -3' miRNA: 3'- -CUCGUGUACUGGCACa---UGCCGaagau -5' |
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25518 | 3' | -52.6 | NC_005337.1 | + | 68916 | 0.83 | 0.216923 |
Target: 5'- aAGCGCGUGugCGUGUGCGGCaUCg- -3' miRNA: 3'- cUCGUGUACugGCACAUGCCGaAGau -5' |
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25518 | 3' | -52.6 | NC_005337.1 | + | 124051 | 1.06 | 0.006579 |
Target: 5'- gGAGCACAUGACCGUGUACGGCUUCUAc -3' miRNA: 3'- -CUCGUGUACUGGCACAUGCCGAAGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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