Results 81 - 83 of 83 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25521 | 3' | -55.6 | NC_005337.1 | + | 53255 | 0.7 | 0.72377 |
Target: 5'- cAGaUAC-GGcCCGUGGA-CGUGGACGCc -3' miRNA: 3'- cUC-AUGaCC-GGCGCCUaGUACCUGCG- -5' |
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25521 | 3' | -55.6 | NC_005337.1 | + | 35757 | 0.7 | 0.72377 |
Target: 5'- ---cGCUGGgCGCgaccgGGAUCGUGG-CGCg -3' miRNA: 3'- cucaUGACCgGCG-----CCUAGUACCuGCG- -5' |
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25521 | 3' | -55.6 | NC_005337.1 | + | 122770 | 1.12 | 0.001745 |
Target: 5'- gGAGUACUGGCCGCGGAUCAUGGACGCg -3' miRNA: 3'- -CUCAUGACCGGCGCCUAGUACCUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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