Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25523 | 3' | -52.9 | NC_005337.1 | + | 98329 | 0.66 | 0.96497 |
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Target: 5'- -cGAGCCGcaacggcacgGACUUCGUCAcgcacguggacgGGCGGa -3' miRNA: 3'- uuCUUGGCaa--------CUGAGGCAGU------------UCGCCa -5' |
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| 25523 | 3' | -52.9 | NC_005337.1 | + | 78033 | 0.67 | 0.935314 |
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Target: 5'- uAGAG--GUUGuuCUCCGUCAGGUGGUu -3' miRNA: 3'- uUCUUggCAACu-GAGGCAGUUCGCCA- -5' |
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| 25523 | 3' | -52.9 | NC_005337.1 | + | 81476 | 0.67 | 0.930071 |
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Target: 5'- cAGGuccGCCGUggugGGCUCCGuguUCAcGCGGUu -3' miRNA: 3'- uUCU---UGGCAa---CUGAGGC---AGUuCGCCA- -5' |
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| 25523 | 3' | -52.9 | NC_005337.1 | + | 14823 | 0.68 | 0.886213 |
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Target: 5'- cGAGGacGCCGUggugGGCguccuuUCCGcCGAGCGGUg -3' miRNA: 3'- -UUCU--UGGCAa---CUG------AGGCaGUUCGCCA- -5' |
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| 25523 | 3' | -52.9 | NC_005337.1 | + | 131427 | 0.69 | 0.871454 |
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Target: 5'- cGGcuCCGcgGACUCCGUCuccGCGGa -3' miRNA: 3'- uUCuuGGCaaCUGAGGCAGuu-CGCCa -5' |
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| 25523 | 3' | -52.9 | NC_005337.1 | + | 73794 | 0.7 | 0.803745 |
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Target: 5'- cGGGACuCGUgGGCUCCGUCAAGgacUGGa -3' miRNA: 3'- uUCUUG-GCAaCUGAGGCAGUUC---GCCa -5' |
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| 25523 | 3' | -52.9 | NC_005337.1 | + | 98966 | 0.72 | 0.704758 |
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Target: 5'- cGGcGCCGUcuucGACUCCG-CGAGCGGg -3' miRNA: 3'- uUCuUGGCAa---CUGAGGCaGUUCGCCa -5' |
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| 25523 | 3' | -52.9 | NC_005337.1 | + | 66053 | 0.76 | 0.495794 |
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Target: 5'- cGGAACCcg-GACgCCGUCAGGCGGg -3' miRNA: 3'- uUCUUGGcaaCUGaGGCAGUUCGCCa -5' |
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| 25523 | 3' | -52.9 | NC_005337.1 | + | 120730 | 1.05 | 0.008465 |
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Target: 5'- gAAGAACCGUUGACUCCGUCAAGCGGUu -3' miRNA: 3'- -UUCUUGGCAACUGAGGCAGUUCGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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