Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25535 | 5' | -55.9 | NC_005337.1 | + | 31917 | 0.7 | 0.668432 |
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Target: 5'- --gCCaGGUGcgCGGCCucGGGGGCAUGUc -3' miRNA: 3'- caaGGaCCACa-GCCGG--CCUCUGUACG- -5' |
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| 25535 | 5' | -55.9 | NC_005337.1 | + | 95589 | 0.71 | 0.616928 |
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Target: 5'- -gUCCgGG-GUCGGCCGGGuGCA-GCa -3' miRNA: 3'- caAGGaCCaCAGCCGGCCUcUGUaCG- -5' |
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| 25535 | 5' | -55.9 | NC_005337.1 | + | 92562 | 0.74 | 0.439707 |
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Target: 5'- -cUCCUGGUucuucgagGaCGGCCGGcuccGGACGUGCu -3' miRNA: 3'- caAGGACCA--------CaGCCGGCC----UCUGUACG- -5' |
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| 25535 | 5' | -55.9 | NC_005337.1 | + | 130948 | 0.75 | 0.430661 |
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Target: 5'- cGUUCCUGGacgccgGcgUGGCCGuGGACGUGCg -3' miRNA: 3'- -CAAGGACCa-----Ca-GCCGGCcUCUGUACG- -5' |
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| 25535 | 5' | -55.9 | NC_005337.1 | + | 18677 | 0.77 | 0.316427 |
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Target: 5'- -aUCCgagGGccgGUCGGUCGGAGACA-GCa -3' miRNA: 3'- caAGGa--CCa--CAGCCGGCCUCUGUaCG- -5' |
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| 25535 | 5' | -55.9 | NC_005337.1 | + | 115452 | 1.12 | 0.001651 |
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Target: 5'- gGUUCCUGGUGUCGGCCGGAGACAUGCg -3' miRNA: 3'- -CAAGGACCACAGCCGGCCUCUGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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