miRNA display CGI


Results 1 - 20 of 21 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
25537 3' -49.8 NC_005337.1 + 89910 0.66 0.996517
Target:  5'- uGGGCGgcgGGGcGUcgccccacUCCUCAcAGAGGGccGCg -3'
miRNA:   3'- -CCUGUa--UCC-CA--------AGGAGU-UCUCCUa-CG- -5'
25537 3' -49.8 NC_005337.1 + 81561 0.66 0.996517
Target:  5'- aGGACAUGuacGGGUcguacUCCUgcgcguacacCAGGAuGGUGCa -3'
miRNA:   3'- -CCUGUAU---CCCA-----AGGA----------GUUCUcCUACG- -5'
25537 3' -49.8 NC_005337.1 + 109381 0.66 0.996459
Target:  5'- uGGACAauaAGGcuaugucaacacuGUUCUUCGAGAagcuguucGGGUGCa -3'
miRNA:   3'- -CCUGUa--UCC-------------CAAGGAGUUCU--------CCUACG- -5'
25537 3' -49.8 NC_005337.1 + 117806 0.66 0.996158
Target:  5'- uGGGCAUGaacggcguggcgcccGGGgaCC-CGGGcGGGGUGCg -3'
miRNA:   3'- -CCUGUAU---------------CCCaaGGaGUUC-UCCUACG- -5'
25537 3' -49.8 NC_005337.1 + 100530 0.66 0.995201
Target:  5'- cGGAgGUGGGGgaUgUCuacGGGGUGCu -3'
miRNA:   3'- -CCUgUAUCCCaaGgAGuucUCCUACG- -5'
25537 3' -49.8 NC_005337.1 + 33205 0.66 0.995201
Target:  5'- cGACGgcGGGgcgcgCCUCucAGAGGAguucGCg -3'
miRNA:   3'- cCUGUauCCCaa---GGAGu-UCUCCUa---CG- -5'
25537 3' -49.8 NC_005337.1 + 19851 0.66 0.995126
Target:  5'- cGGACGUcg---UCCUCcgugauguccaugAAGGGGAUGCa -3'
miRNA:   3'- -CCUGUAucccaAGGAG-------------UUCUCCUACG- -5'
25537 3' -49.8 NC_005337.1 + 10679 0.67 0.991363
Target:  5'- cGGCGgcGGGUucUCCUCGA-AGGcgGCc -3'
miRNA:   3'- cCUGUauCCCA--AGGAGUUcUCCuaCG- -5'
25537 3' -49.8 NC_005337.1 + 68227 0.67 0.990099
Target:  5'- cGGuCGcuccccUGGGGcUCCUCcacgAAGAGGAcgUGCa -3'
miRNA:   3'- -CCuGU------AUCCCaAGGAG----UUCUCCU--ACG- -5'
25537 3' -49.8 NC_005337.1 + 1611 0.67 0.988696
Target:  5'- gGGGCGUGaucccGUUCCUCGaucGGAGGucgAUGCg -3'
miRNA:   3'- -CCUGUAUcc---CAAGGAGU---UCUCC---UACG- -5'
25537 3' -49.8 NC_005337.1 + 34381 0.68 0.979237
Target:  5'- uGGACuccgcgGUGGGGUUCaC-CAGGcAGGAcGCg -3'
miRNA:   3'- -CCUG------UAUCCCAAG-GaGUUC-UCCUaCG- -5'
25537 3' -49.8 NC_005337.1 + 20999 0.68 0.979002
Target:  5'- uGGGCAUGGuGUUCUUgcuccggCAGGAGGG-GCa -3'
miRNA:   3'- -CCUGUAUCcCAAGGA-------GUUCUCCUaCG- -5'
25537 3' -49.8 NC_005337.1 + 3831 0.69 0.964883
Target:  5'- uGGACAUGaaccGGcUCgaCAGGAGGAUGUc -3'
miRNA:   3'- -CCUGUAUc---CCaAGgaGUUCUCCUACG- -5'
25537 3' -49.8 NC_005337.1 + 71107 0.69 0.964883
Target:  5'- cGGGCGgcGGGcucagcacgUCCUCGGGcGGcgGCa -3'
miRNA:   3'- -CCUGUauCCCa--------AGGAGUUCuCCuaCG- -5'
25537 3' -49.8 NC_005337.1 + 132470 0.7 0.953516
Target:  5'- uGACAaagAGGGUUCCcgugcggcggCAGGAGGGacugGCg -3'
miRNA:   3'- cCUGUa--UCCCAAGGa---------GUUCUCCUa---CG- -5'
25537 3' -49.8 NC_005337.1 + 74483 0.72 0.914465
Target:  5'- uGGACgcguacaucgugcggGUAGGGUcCCUgAAGAGGcUGUa -3'
miRNA:   3'- -CCUG---------------UAUCCCAaGGAgUUCUCCuACG- -5'
25537 3' -49.8 NC_005337.1 + 127660 0.73 0.870016
Target:  5'- uGGACGUGGaGGUg-CUCGAGcuGAUGCu -3'
miRNA:   3'- -CCUGUAUC-CCAagGAGUUCucCUACG- -5'
25537 3' -49.8 NC_005337.1 + 125484 0.73 0.862169
Target:  5'- -aGCAgcaGGGGUUCCUCGgcaucgagguGGAGGucGUGCg -3'
miRNA:   3'- ccUGUa--UCCCAAGGAGU----------UCUCC--UACG- -5'
25537 3' -49.8 NC_005337.1 + 83933 0.78 0.637084
Target:  5'- cGGCGUGGGGUUCUUgGAGAagcGGAuuUGCg -3'
miRNA:   3'- cCUGUAUCCCAAGGAgUUCU---CCU--ACG- -5'
25537 3' -49.8 NC_005337.1 + 37015 0.78 0.604936
Target:  5'- gGGACGUGGGG-UCg--AAGAGGAUGCu -3'
miRNA:   3'- -CCUGUAUCCCaAGgagUUCUCCUACG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.