Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25538 | 3' | -55.9 | NC_005337.1 | + | 28541 | 0.69 | 0.765474 |
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Target: 5'- gUCCUCGGAguagaGGAUccggACGCGCCcagaggcGGUCUc -3' miRNA: 3'- -GGGAGCCU-----UCUAca--UGCGCGG-------CCAGA- -5' |
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| 25538 | 3' | -55.9 | NC_005337.1 | + | 33178 | 0.7 | 0.7279 |
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Target: 5'- uCCUUCGaGAGGAUG-AUGUGCaUGGUCg -3' miRNA: 3'- -GGGAGC-CUUCUACaUGCGCG-GCCAGa -5' |
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| 25538 | 3' | -55.9 | NC_005337.1 | + | 111045 | 0.7 | 0.7279 |
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Target: 5'- cCCCU-GGAGGAUG-ACGCGgCGGa-- -3' miRNA: 3'- -GGGAgCCUUCUACaUGCGCgGCCaga -5' |
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| 25538 | 3' | -55.9 | NC_005337.1 | + | 48701 | 0.7 | 0.718042 |
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Target: 5'- cCUCUCGGcagaccagAAGgcGUACGUGCCGGggCUu -3' miRNA: 3'- -GGGAGCC--------UUCuaCAUGCGCGGCCa-GA- -5' |
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| 25538 | 3' | -55.9 | NC_005337.1 | + | 99416 | 0.71 | 0.65768 |
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Target: 5'- aCgUCGGAGGAUG-GCGCGCCGccgCa -3' miRNA: 3'- gGgAGCCUUCUACaUGCGCGGCca-Ga -5' |
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| 25538 | 3' | -55.9 | NC_005337.1 | + | 108948 | 0.72 | 0.596568 |
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Target: 5'- aCCUCGucguuGGGGUcGUacACGCGCCGGUCg -3' miRNA: 3'- gGGAGCc----UUCUA-CA--UGCGCGGCCAGa -5' |
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| 25538 | 3' | -55.9 | NC_005337.1 | + | 58714 | 0.72 | 0.596568 |
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Target: 5'- gCCgUCGGcagguAGAUGgGCGCGCCGG-Cg -3' miRNA: 3'- -GGgAGCCu----UCUACaUGCGCGGCCaGa -5' |
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| 25538 | 3' | -55.9 | NC_005337.1 | + | 128629 | 0.72 | 0.576325 |
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Target: 5'- cCCCUCGGuuucccugcuGGAUauccUGCGCgGCCGGUCg -3' miRNA: 3'- -GGGAGCCu---------UCUAc---AUGCG-CGGCCAGa -5' |
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| 25538 | 3' | -55.9 | NC_005337.1 | + | 95837 | 0.75 | 0.450602 |
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Target: 5'- gCCUCGGGcguGAUG-ACGCGCCccuGGUCg -3' miRNA: 3'- gGGAGCCUu--CUACaUGCGCGG---CCAGa -5' |
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| 25538 | 3' | -55.9 | NC_005337.1 | + | 114217 | 1.09 | 0.003172 |
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Target: 5'- uCCCUCGGAAGAUGUACGCGCCGGUCUc -3' miRNA: 3'- -GGGAGCCUUCUACAUGCGCGGCCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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