Results 1 - 20 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25549 | 3' | -60.4 | NC_005337.1 | + | 109073 | 1.06 | 0.001432 |
Target: 5'- gUUCGAGUCCCGCGCGAUGCCGCUGGAg -3' miRNA: 3'- -AAGCUCAGGGCGCGCUACGGCGACCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 73894 | 0.75 | 0.220338 |
Target: 5'- aUCGAGgucugCCUGgGCagcgaGAUGCCGCUGGGg -3' miRNA: 3'- aAGCUCa----GGGCgCG-----CUACGGCGACCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 17040 | 0.73 | 0.279245 |
Target: 5'- gUCGcGUUCCGCGCGGUcugcacguuCCGCUGGAu -3' miRNA: 3'- aAGCuCAGGGCGCGCUAc--------GGCGACCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 22056 | 0.73 | 0.292434 |
Target: 5'- -cCGAGUUCCGCGCGGUGaagCGcCUGGc -3' miRNA: 3'- aaGCUCAGGGCGCGCUACg--GC-GACCu -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 94326 | 0.71 | 0.365624 |
Target: 5'- gUCGAGcgCCCGCGCGAggcgcccGCCGCg--- -3' miRNA: 3'- aAGCUCa-GGGCGCGCUa------CGGCGaccu -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 84942 | 0.71 | 0.381699 |
Target: 5'- aUCGAGUugCCCucCGCG-UGUCGCUGGAu -3' miRNA: 3'- aAGCUCA--GGGc-GCGCuACGGCGACCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 122187 | 0.7 | 0.415232 |
Target: 5'- cUCGGGUCCaCGCGCGAgGgCG-UGGAc -3' miRNA: 3'- aAGCUCAGG-GCGCGCUaCgGCgACCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 127606 | 0.7 | 0.450511 |
Target: 5'- -cCGAGUCgUGCuGUGGgugcacGCCGCUGGAu -3' miRNA: 3'- aaGCUCAGgGCG-CGCUa-----CGGCGACCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 53895 | 0.7 | 0.459586 |
Target: 5'- gUCGGGgcggcCCCGCugGCGGUGgagaCGCUGGAc -3' miRNA: 3'- aAGCUCa----GGGCG--CGCUACg---GCGACCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 43916 | 0.69 | 0.478021 |
Target: 5'- --aGAGg-CCGCGCGuaacgGCCGCUGGc -3' miRNA: 3'- aagCUCagGGCGCGCua---CGGCGACCu -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 42761 | 0.69 | 0.487375 |
Target: 5'- -gCGGGUCCCGCGCGuucGUG-UGCUcGGGc -3' miRNA: 3'- aaGCUCAGGGCGCGC---UACgGCGA-CCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 120448 | 0.69 | 0.487375 |
Target: 5'- -cCGAGUCCCugcgggGCGCGcucgagcGCgCGCUGGAu -3' miRNA: 3'- aaGCUCAGGG------CGCGCua-----CG-GCGACCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 73303 | 0.69 | 0.487375 |
Target: 5'- cUUCGAGUCCCaGCGCGuggGCgCGCg--- -3' miRNA: 3'- -AAGCUCAGGG-CGCGCua-CG-GCGaccu -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 101232 | 0.69 | 0.506334 |
Target: 5'- --gGAGgcgUCCGCGCGcGUGCucgCGCUGGAc -3' miRNA: 3'- aagCUCa--GGGCGCGC-UACG---GCGACCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 75391 | 0.69 | 0.515931 |
Target: 5'- gUCGAcGUCuuggCCGCGCgGAUGCUGC-GGAc -3' miRNA: 3'- aAGCU-CAG----GGCGCG-CUACGGCGaCCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 107936 | 0.68 | 0.525601 |
Target: 5'- --aGGG-CCCGCugauGCGGcUGCUGCUGGAc -3' miRNA: 3'- aagCUCaGGGCG----CGCU-ACGGCGACCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 96316 | 0.68 | 0.534362 |
Target: 5'- -cCGGGaaggugaccugccUCCCGCugGCGgcGCUGCUGGAc -3' miRNA: 3'- aaGCUC-------------AGGGCG--CGCuaCGGCGACCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 54869 | 0.68 | 0.535339 |
Target: 5'- aUCGAGaUgCCGCGCc--GCCGCgGGAg -3' miRNA: 3'- aAGCUC-AgGGCGCGcuaCGGCGaCCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 64024 | 0.68 | 0.545139 |
Target: 5'- -gCGAGUCCaaCGCGCaGAUgGCCGCcuuccuccucaUGGAg -3' miRNA: 3'- aaGCUCAGG--GCGCG-CUA-CGGCG-----------ACCU- -5' |
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25549 | 3' | -60.4 | NC_005337.1 | + | 69152 | 0.68 | 0.564906 |
Target: 5'- -gCGcGUCUCGCGCG-UGCUGCUGc- -3' miRNA: 3'- aaGCuCAGGGCGCGCuACGGCGACcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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