Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25559 | 5' | -54.3 | NC_005337.1 | + | 67189 | 0.67 | 0.920341 |
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Target: 5'- cGGCGccGGGCUgcaagGUgcucaucaccaccaUGGACGGCGACUa -3' miRNA: 3'- -CCGCa-CUCGGa----CA--------------ACCUGCUGUUGGa -5' |
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| 25559 | 5' | -54.3 | NC_005337.1 | + | 56143 | 0.67 | 0.922041 |
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Target: 5'- cGGCGUGcAGCg----GGGCGGCAagGCCg -3' miRNA: 3'- -CCGCAC-UCGgacaaCCUGCUGU--UGGa -5' |
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| 25559 | 5' | -54.3 | NC_005337.1 | + | 46252 | 0.66 | 0.927546 |
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Target: 5'- aGCGUGA-CCUGgauggUGGGcCGGCAcACCa -3' miRNA: 3'- cCGCACUcGGACa----ACCU-GCUGU-UGGa -5' |
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| 25559 | 5' | -54.3 | NC_005337.1 | + | 84682 | 0.66 | 0.932803 |
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Target: 5'- cGGaCG-GGGCCgUGUUcgaGGugGACGACUUc -3' miRNA: 3'- -CC-GCaCUCGG-ACAA---CCugCUGUUGGA- -5' |
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| 25559 | 5' | -54.3 | NC_005337.1 | + | 28974 | 0.66 | 0.932803 |
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Target: 5'- gGGCGUGcgcAGCgaCUGcgGGcaGCGGCGGCCg -3' miRNA: 3'- -CCGCAC---UCG--GACaaCC--UGCUGUUGGa -5' |
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| 25559 | 5' | -54.3 | NC_005337.1 | + | 55950 | 0.66 | 0.932803 |
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Target: 5'- cGCGUGGucGCCgccaacgUGGACaGCGACCUc -3' miRNA: 3'- cCGCACU--CGGaca----ACCUGcUGUUGGA- -5' |
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| 25559 | 5' | -54.3 | NC_005337.1 | + | 102298 | 0.66 | 0.932803 |
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Target: 5'- uGGUGcaGuuCCUGgcGGACGugAGCCUg -3' miRNA: 3'- -CCGCa-CucGGACaaCCUGCugUUGGA- -5' |
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| 25559 | 5' | -54.3 | NC_005337.1 | + | 65683 | 0.66 | 0.937814 |
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Target: 5'- aGGCGcgGAGuuugucCCUGUUGG-UGcACAGCCg -3' miRNA: 3'- -CCGCa-CUC------GGACAACCuGC-UGUUGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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