Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 25568 | 5' | -51.2 | NC_005337.1 | + | 15193 | 0.74 | 0.702909 |
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Target: 5'- -gUGUUCCAGCAU-AGCGUccaGGCGCUCg -3' miRNA: 3'- cgACGAGGUUGUAgUUGCA---CUGCGAG- -5' |
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| 25568 | 5' | -51.2 | NC_005337.1 | + | 99342 | 0.75 | 0.639077 |
|
Target: 5'- cGCUGCUUCAugcgcgugaaggGCAUCGACuUGACGCcCg -3' miRNA: 3'- -CGACGAGGU------------UGUAGUUGcACUGCGaG- -5' |
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| 25568 | 5' | -51.2 | NC_005337.1 | + | 48258 | 0.76 | 0.606949 |
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Target: 5'- aGgUGCUCCA---UCGGCGUGACGCa- -3' miRNA: 3'- -CgACGAGGUuguAGUUGCACUGCGag -5' |
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| 25568 | 5' | -51.2 | NC_005337.1 | + | 110482 | 0.78 | 0.510459 |
|
Target: 5'- uGCgGCgcagguaccgggCCAGC-UCGACGUGGCGCUCg -3' miRNA: 3'- -CGaCGa-----------GGUUGuAGUUGCACUGCGAG- -5' |
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| 25568 | 5' | -51.2 | NC_005337.1 | + | 99083 | 1.13 | 0.003574 |
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Target: 5'- aGCUGCUCCAACAUCAACGUGACGCUCg -3' miRNA: 3'- -CGACGAGGUUGUAGUUGCACUGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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