miRNA: 3'- -CGACGAgGUU-GUA-GUUGCaCUGCGAg -5'
miRNA: 3'- -CGACGA-GGUUGuaGUUGCacUGCGaG- -5'
miRNA: 3'- -CGACGA-GGUUGuaGUUGCacUGCGaG- -5'
miRNA: 3'- -CGACga--GGUUGU------AGU---UGCACUGCGAG- -5'
miRNA: 3'- -CGACga--GGUUGU------AGU---UGCACUGCGAG- -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 25568 | 5' | -51.2 | NC_005337.1 | + | 132278 | 0.71 | 0.86351 |
|
Target: 5'- uGCUGCUgCGGuCGUcCGACGcGACGCUg -3' miRNA: 3'- -CGACGAgGUU-GUA-GUUGCaCUGCGAg -5' |
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| 25568 | 5' | -51.2 | NC_005337.1 | + | 133168 | 0.66 | 0.979591 |
|
Target: 5'- gGCUGCUcCCGGCggCGGCGcuGCGC-Cg -3' miRNA: 3'- -CGACGA-GGUUGuaGUUGCacUGCGaG- -5' |
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| 25568 | 5' | -51.2 | NC_005337.1 | + | 133168 | 0.66 | 0.979591 |
|
Target: 5'- gGCUGCUcCCGGCggCGGCGcuGCGC-Cg -3' miRNA: 3'- -CGACGA-GGUUGuaGUUGCacUGCGaG- -5' |
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| 25568 | 5' | -51.2 | NC_005337.1 | + | 133659 | 0.68 | 0.95816 |
|
Target: 5'- cGCUGgacgCCGACGcggaguUCGccuGCGUGGCGUUCg -3' miRNA: 3'- -CGACga--GGUUGU------AGU---UGCACUGCGAG- -5' |
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| 25568 | 5' | -51.2 | NC_005337.1 | + | 133659 | 0.68 | 0.95816 |
|
Target: 5'- cGCUGgacgCCGACGcggaguUCGccuGCGUGGCGUUCg -3' miRNA: 3'- -CGACga--GGUUGU------AGU---UGCACUGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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