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miRNA: 3'- gaCCGGUa-GCGG--CCGUGGAaGGCCCu -5'
miRNA: 3'- gACCGguAGCGGCCGUgGAAGGC-C-CU- -5'
miRNA: 3'- gACCGguAGCGGCCGUGGaa-------GGCCCU- -5'
miRNA: 3'- -GACCGGUAGCGGCCGUGGAAGGCCCU- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 25573 | 5' | -61.4 | NC_005337.1 | + | 91883 | 0.74 | 0.233417 |
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Target: 5'- -aGGCCGcgCGCCgcGGCGCCU-CCGGGu -3' miRNA: 3'- gaCCGGUa-GCGG--CCGUGGAaGGCCCu -5' |
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| 25573 | 5' | -61.4 | NC_005337.1 | + | 133688 | 0.74 | 0.244664 |
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Target: 5'- gUGGCguUCGCCGGCGgCUUCCGcGcGGc -3' miRNA: 3'- gACCGguAGCGGCCGUgGAAGGC-C-CU- -5' |
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| 25573 | 5' | -61.4 | NC_005337.1 | + | 97725 | 0.76 | 0.179126 |
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Target: 5'- gUGGC--UCGCCGGCGCCgcccagccgCCGGGGa -3' miRNA: 3'- gACCGguAGCGGCCGUGGaa-------GGCCCU- -5' |
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| 25573 | 5' | -61.4 | NC_005337.1 | + | 97614 | 1.08 | 0.001009 |
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Target: 5'- gCUGGCCAUCGCCGGCACCUUCCGGGAc -3' miRNA: 3'- -GACCGGUAGCGGCCGUGGAAGGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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