miRNA: 3'- gaCCGGUAG--CGgCCG--------UGGAA-GGC-CCU- -5'
miRNA: 3'- gacCGGUAGCGG----------CCGUGGaa------GGCCCU- -5'
miRNA: 3'- gaCCGGUA--GCGGCCGUgGAaGGCccu -5'
miRNA: 3'- gaCCGGUAGcGGCCG----UGGAa----GGC-CCU- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25573 | 5' | -61.4 | NC_005337.1 | + | 35748 | 0.66 | 0.662258 |
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Target: 5'- -cGGCCGUCggGCgGGCuccccccgGCCUUgCCGcGGAa -3' miRNA: 3'- gaCCGGUAG--CGgCCG--------UGGAA-GGC-CCU- -5' |
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| 25573 | 5' | -61.4 | NC_005337.1 | + | 93606 | 0.66 | 0.662258 |
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Target: 5'- --cGCCGUCGCCgcgccuagagGGCGCCccacugcgUCGGGAc -3' miRNA: 3'- gacCGGUAGCGG----------CCGUGGaa------GGCCCU- -5' |
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| 25573 | 5' | -61.4 | NC_005337.1 | + | 81237 | 0.66 | 0.662258 |
|
Target: 5'- -aGGCCGUugCGCCGGCAgCUcCCGc-- -3' miRNA: 3'- gaCCGGUA--GCGGCCGUgGAaGGCccu -5' |
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| 25573 | 5' | -61.4 | NC_005337.1 | + | 103990 | 0.66 | 0.662258 |
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Target: 5'- -cGGCCGUC-CUGGCgcucACCUgcgcgCCGuGGAc -3' miRNA: 3'- gaCCGGUAGcGGCCG----UGGAa----GGC-CCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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