Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25573 | 5' | -61.4 | NC_005337.1 | + | 10777 | 0.66 | 0.612532 |
Target: 5'- -cGGCCAUCGUCaGGCggacgACCgUCCcGGAc -3' miRNA: 3'- gaCCGGUAGCGG-CCG-----UGGaAGGcCCU- -5' |
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25573 | 5' | -61.4 | NC_005337.1 | + | 3327 | 0.67 | 0.572956 |
Target: 5'- -cGGCCA-CGCCGGCG---UCCaGGAa -3' miRNA: 3'- gaCCGGUaGCGGCCGUggaAGGcCCU- -5' |
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25573 | 5' | -61.4 | NC_005337.1 | + | 1093 | 0.68 | 0.505424 |
Target: 5'- -gGGCUAcaGCCGGCgcagcGCCgccgCCGGGAg -3' miRNA: 3'- gaCCGGUagCGGCCG-----UGGaa--GGCCCU- -5' |
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25573 | 5' | -61.4 | NC_005337.1 | + | 1093 | 0.68 | 0.505424 |
Target: 5'- -gGGCUAcaGCCGGCgcagcGCCgccgCCGGGAg -3' miRNA: 3'- gaCCGGUagCGGCCG-----UGGaa--GGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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