Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
256 | 5' | -64.7 | AC_000008.1 | + | 26799 | 0.73 | 0.061332 |
Target: 5'- -gCGCCugCGaaacuaCGCCGucuucGCCCGGCACg -3' miRNA: 3'- caGCGGugGCg-----GCGGC-----UGGGCCGUGa -5' |
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256 | 5' | -64.7 | AC_000008.1 | + | 26961 | 0.67 | 0.168957 |
Target: 5'- cUCGCCGCCaCCGUggagCGAgCCGGaCGCg -3' miRNA: 3'- cAGCGGUGGcGGCG----GCUgGGCC-GUGa -5' |
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256 | 5' | -64.7 | AC_000008.1 | + | 33229 | 0.66 | 0.208162 |
Target: 5'- -cUGCUGCCGCCGCCGcUCCGuccuGCAg- -3' miRNA: 3'- caGCGGUGGCGGCGGCuGGGC----CGUga -5' |
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256 | 5' | -64.7 | AC_000008.1 | + | 33704 | 0.7 | 0.109853 |
Target: 5'- -cUGCUccuGCCGCCGCCG-CCUGGCu-- -3' miRNA: 3'- caGCGG---UGGCGGCGGCuGGGCCGuga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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