miRNA: 3'- caGCGGUGGCGGCGGCuG-GGCCGUGa -5'
miRNA: 3'- caGCGgUGgCGGCGGCUGGGCCGUga -5'
miRNA: 3'- caGCGGUGGCGGCGGcuGGG-CCGUGa -5'
miRNA: 3'- -CAGCGGUGGCGGCGGCUGGGCCGUGA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 256 | 5' | -64.7 | AC_000008.1 | + | 9185 | 0.73 | 0.059632 |
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Target: 5'- -cUGCCGCUGCCGCUGcCgCCGGUGCa -3' miRNA: 3'- caGCGGUGGCGGCGGCuG-GGCCGUGa -5' |
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| 256 | 5' | -64.7 | AC_000008.1 | + | 11487 | 0.74 | 0.044964 |
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Target: 5'- -aCGUgGCgGCCGCCGACCUGGUAa- -3' miRNA: 3'- caGCGgUGgCGGCGGCUGGGCCGUga -5' |
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| 256 | 5' | -64.7 | AC_000008.1 | + | 11994 | 0.76 | 0.034818 |
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Target: 5'- -cCGUCGCCGCCGCCucCCCgGGCGCc -3' miRNA: 3'- caGCGGUGGCGGCGGcuGGG-CCGUGa -5' |
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| 256 | 5' | -64.7 | AC_000008.1 | + | 16180 | 1.07 | 0.000101 |
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Target: 5'- cGUCGCCACCGCCGCCGACCCGGCACUg -3' miRNA: 3'- -CAGCGGUGGCGGCGGCUGGGCCGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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