Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25617 | 5' | -52.8 | NC_005337.1 | + | 117922 | 0.71 | 0.816693 |
Target: 5'- uCGAgGGCGCCGaagGCgCUGacgAGGACGa -3' miRNA: 3'- -GCU-CCGCGGCaa-CG-GAUaaaUCCUGC- -5' |
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25617 | 5' | -52.8 | NC_005337.1 | + | 15493 | 0.71 | 0.77791 |
Target: 5'- aCGAcGGCGCCGccGCCggcgucaucUAGGACGu -3' miRNA: 3'- -GCU-CCGCGGCaaCGGauaa-----AUCCUGC- -5' |
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25617 | 5' | -52.8 | NC_005337.1 | + | 74643 | 0.72 | 0.760504 |
Target: 5'- gCGGGGCGCCGcUGCCcg---GGGAg- -3' miRNA: 3'- -GCUCCGCGGCaACGGauaaaUCCUgc -5' |
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25617 | 5' | -52.8 | NC_005337.1 | + | 33677 | 0.75 | 0.595324 |
Target: 5'- gCGAGGCGCCGagGCCggc---GGGCGc -3' miRNA: 3'- -GCUCCGCGGCaaCGGauaaauCCUGC- -5' |
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25617 | 5' | -52.8 | NC_005337.1 | + | 70610 | 1.09 | 0.005412 |
Target: 5'- cCGAGGCGCCGUUGCCUAUUUAGGACGc -3' miRNA: 3'- -GCUCCGCGGCAACGGAUAAAUCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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