Results 141 - 149 of 149 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25647 | 5' | -54.9 | NC_005337.1 | + | 25029 | 0.74 | 0.516747 |
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Target: 5'- gUGCGCgCgGCGGACaccGAGGAGCUGCu -3' miRNA: 3'- -ACGCG-GgCGUCUGgcaCUUCUUGAUG- -5' |
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| 25647 | 5' | -54.9 | NC_005337.1 | + | 123616 | 0.74 | 0.496921 |
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Target: 5'- cGCGCUCGCGGGugcugacgcUCGUGGAGcuCUGCa -3' miRNA: 3'- aCGCGGGCGUCU---------GGCACUUCuuGAUG- -5' |
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| 25647 | 5' | -54.9 | NC_005337.1 | + | 90404 | 0.74 | 0.47648 |
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Target: 5'- cGCGUCgCGCAcguucguGGCCGUGAccauGAACUGCa -3' miRNA: 3'- aCGCGG-GCGU-------CUGGCACUu---CUUGAUG- -5' |
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| 25647 | 5' | -54.9 | NC_005337.1 | + | 47688 | 0.75 | 0.458352 |
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Target: 5'- gUGCGCCUGCGGuCCGUGuccGAGAucguggcCUACg -3' miRNA: 3'- -ACGCGGGCGUCuGGCAC---UUCUu------GAUG- -5' |
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| 25647 | 5' | -54.9 | NC_005337.1 | + | 112398 | 0.76 | 0.412495 |
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Target: 5'- aGCGCCgCGCAGGCCGUGcgguAGGcCUGg -3' miRNA: 3'- aCGCGG-GCGUCUGGCACu---UCUuGAUg -5' |
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| 25647 | 5' | -54.9 | NC_005337.1 | + | 117844 | 0.77 | 0.353294 |
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Target: 5'- gUGCGCaCGCAGGCgGUGggGGACa-- -3' miRNA: 3'- -ACGCGgGCGUCUGgCACuuCUUGaug -5' |
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| 25647 | 5' | -54.9 | NC_005337.1 | + | 112591 | 0.79 | 0.272966 |
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Target: 5'- gGUGCCCGC-GugCGUGucGGACUGCu -3' miRNA: 3'- aCGCGGGCGuCugGCACuuCUUGAUG- -5' |
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| 25647 | 5' | -54.9 | NC_005337.1 | + | 115361 | 0.8 | 0.247614 |
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Target: 5'- cGUGCCCGCGGACgugGUGggGAGC-ACg -3' miRNA: 3'- aCGCGGGCGUCUGg--CACuuCUUGaUG- -5' |
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| 25647 | 5' | -54.9 | NC_005337.1 | + | 53525 | 1.1 | 0.002391 |
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Target: 5'- cUGCGCCCGCAGACCGUGAAGAACUACg -3' miRNA: 3'- -ACGCGGGCGUCUGGCACUUCUUGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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