Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25657 | 5' | -57 | NC_005337.1 | + | 117820 | 0.66 | 0.824279 |
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Target: 5'- gUGGc-GCCCGGGgaCCCgGGCGGGGUg -3' miRNA: 3'- -ACCucUGGGCCUa-GGGaCUGUUCCAg -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 47645 | 0.66 | 0.831041 |
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Target: 5'- cGGAGuucgcugugacgaGCCCGGcgCCCggcaugcUGGCGGGcGUCc -3' miRNA: 3'- aCCUC-------------UGGGCCuaGGG-------ACUGUUC-CAG- -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 38296 | 0.66 | 0.832714 |
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Target: 5'- gGGGGACuCCGuGUCCaagGugGAGGUg -3' miRNA: 3'- aCCUCUG-GGCcUAGGga-CugUUCCAg -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 111692 | 0.66 | 0.836037 |
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Target: 5'- cGGcgacGACCCGGAagaggucgUCCCUGaACcgcgcguccgcggugAAGGUCu -3' miRNA: 3'- aCCu---CUGGGCCU--------AGGGAC-UG---------------UUCCAG- -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 88030 | 0.66 | 0.840965 |
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Target: 5'- aGGAuGugCCGGcgCaCgUGcACGGGGUCg -3' miRNA: 3'- aCCU-CugGGCCuaG-GgAC-UGUUCCAG- -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 82190 | 0.66 | 0.840965 |
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Target: 5'- -cGAGGCUggaGGAUCCgCUGGgGAGGUa -3' miRNA: 3'- acCUCUGGg--CCUAGG-GACUgUUCCAg -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 55780 | 0.66 | 0.849026 |
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Target: 5'- cGGAuauccuacGACaCCgGGAUCCUggugGAgAAGGUCa -3' miRNA: 3'- aCCU--------CUG-GG-CCUAGGGa---CUgUUCCAG- -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 122877 | 0.66 | 0.849026 |
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Target: 5'- cGGA-ACCCGGggCCCUGGCGc---- -3' miRNA: 3'- aCCUcUGGGCCuaGGGACUGUuccag -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 115155 | 0.66 | 0.849026 |
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Target: 5'- cGGAGGCCUGcguccgacaGAagcUCCCUGACAucguGGa- -3' miRNA: 3'- aCCUCUGGGC---------CU---AGGGACUGUu---CCag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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