Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25657 | 5' | -57 | NC_005337.1 | + | 122985 | 0.7 | 0.640649 |
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Target: 5'- gUGGAGACCCGGAaCaugggCgugGGCGAGGg- -3' miRNA: 3'- -ACCUCUGGGCCUaGg----Ga--CUGUUCCag -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 13910 | 0.7 | 0.620127 |
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Target: 5'- cGGcAGGCgcgCCGGGUCCCgaagGACGcguucaugAGGUCg -3' miRNA: 3'- aCC-UCUG---GGCCUAGGGa---CUGU--------UCCAG- -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 96530 | 0.7 | 0.620127 |
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Target: 5'- gGGuuGCUCGGGUCCUUGGgAAGGa- -3' miRNA: 3'- aCCucUGGGCCUAGGGACUgUUCCag -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 122508 | 0.7 | 0.613974 |
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Target: 5'- gGGuGACCCGGAgaccucgcugcuggCCCUGGCGcGG-Cg -3' miRNA: 3'- aCCuCUGGGCCUa-------------GGGACUGUuCCaG- -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 2528 | 0.71 | 0.538978 |
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Target: 5'- cGGAGGCCCGGAcgUCCgGGCcccGGGUg -3' miRNA: 3'- aCCUCUGGGCCUa-GGGaCUGu--UCCAg -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 101718 | 0.72 | 0.519194 |
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Target: 5'- cGGAGGCCCGcacugCCCUGACGugccGGGa- -3' miRNA: 3'- aCCUCUGGGCcua--GGGACUGU----UCCag -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 79775 | 0.73 | 0.461747 |
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Target: 5'- cGGAGGCCCccaggcgcGGGUCCUucacggUGcCGAGGUCg -3' miRNA: 3'- aCCUCUGGG--------CCUAGGG------ACuGUUCCAG- -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 14495 | 0.74 | 0.39075 |
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Target: 5'- cGGGGACCa-GAUCCCUG-CGAGGcUCu -3' miRNA: 3'- aCCUCUGGgcCUAGGGACuGUUCC-AG- -5' |
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| 25657 | 5' | -57 | NC_005337.1 | + | 50448 | 1.1 | 0.001719 |
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Target: 5'- cUGGAGACCCGGAUCCCUGACAAGGUCa -3' miRNA: 3'- -ACCUCUGGGCCUAGGGACUGUUCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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