miRNA: 3'- uCCGUGA--ACG-GGGGUCaGCagGAGCU- -5'
miRNA: 3'- ucCGUGaACGGG-GGUCa-GCAGgAGCU- -5'
miRNA: 3'- -UC-CGUGAACGGg--GGUCAGCAGgAGcu -5'
miRNA: 3'- -UCCGUGaaCGGGGG---------------UCAgCAGgAGCU- -5'
miRNA: 3'- uCCGuGAacGGGGG----------UCAGCAGGAGCU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25678 | 5' | -59 | NC_005337.1 | + | 97176 | 0.66 | 0.743567 |
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Target: 5'- cGGCGCUgcUGCgCUCCGG-CGagCUCGAc -3' miRNA: 3'- uCCGUGA--ACG-GGGGUCaGCagGAGCU- -5' |
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| 25678 | 5' | -59 | NC_005337.1 | + | 79094 | 0.66 | 0.753131 |
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Target: 5'- -cGCGCaUGCCCaCCGGcaCGUCgUCGAc -3' miRNA: 3'- ucCGUGaACGGG-GGUCa-GCAGgAGCU- -5' |
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| 25678 | 5' | -59 | NC_005337.1 | + | 106522 | 0.66 | 0.753131 |
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Target: 5'- uAGcGUGCUUGCUuaaCCAGUCGUCgUCu- -3' miRNA: 3'- -UC-CGUGAACGGg--GGUCAGCAGgAGcu -5' |
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| 25678 | 5' | -59 | NC_005337.1 | + | 62048 | 0.66 | 0.756928 |
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Target: 5'- aAGGCGC--GCCCCCacucgacgaugcccgGGUgGUCgUCGGc -3' miRNA: 3'- -UCCGUGaaCGGGGG---------------UCAgCAGgAGCU- -5' |
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| 25678 | 5' | -59 | NC_005337.1 | + | 81627 | 0.66 | 0.76259 |
|
Target: 5'- uGGC-CU--CCUCCauggucagcaGGUCGUCCUCGAa -3' miRNA: 3'- uCCGuGAacGGGGG----------UCAGCAGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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