Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25685 | 5' | -57.1 | NC_005337.1 | + | 68659 | 0.71 | 0.563314 |
Target: 5'- uUGGGGUGGUugaGCGCGuGcagCAGCGcgcUCUUGg -3' miRNA: 3'- -ACUCCACCG---UGCGC-Ca--GUCGC---AGAAC- -5' |
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25685 | 5' | -57.1 | NC_005337.1 | + | 28153 | 0.75 | 0.337605 |
Target: 5'- aGAGGUcaacGGCACGCGuGUCAGCGa---- -3' miRNA: 3'- aCUCCA----CCGUGCGC-CAGUCGCagaac -5' |
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25685 | 5' | -57.1 | NC_005337.1 | + | 64289 | 0.77 | 0.261292 |
Target: 5'- aUGAGGU-GCACgGCGGUCgcguccGGCGUCUUGc -3' miRNA: 3'- -ACUCCAcCGUG-CGCCAG------UCGCAGAAC- -5' |
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25685 | 5' | -57.1 | NC_005337.1 | + | 37239 | 1.08 | 0.002334 |
Target: 5'- aUGAGGUGGCACGCGGUCAGCGUCUUGc -3' miRNA: 3'- -ACUCCACCGUGCGCCAGUCGCAGAAC- -5' |
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25685 | 5' | -57.1 | NC_005337.1 | + | 39926 | 0.66 | 0.844924 |
Target: 5'- --cGuGUGGCGCGCGcUgAGCGUCa-- -3' miRNA: 3'- acuC-CACCGUGCGCcAgUCGCAGaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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