Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25697 | 5' | -54.3 | NC_005337.1 | + | 5130 | 0.66 | 0.921253 |
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Target: 5'- cGCGGGugucuGGUGUGGAGGuagaggugcAGCgcgGACUUGUc -3' miRNA: 3'- uCGCUU-----UCACACCUCC---------UCG---CUGAGCA- -5' |
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| 25697 | 5' | -54.3 | NC_005337.1 | + | 60282 | 0.67 | 0.915437 |
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Target: 5'- cGCGggGGUG-GGAGuGGGCuugGGgUCGUc -3' miRNA: 3'- uCGCuuUCACaCCUC-CUCG---CUgAGCA- -5' |
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| 25697 | 5' | -54.3 | NC_005337.1 | + | 129606 | 0.67 | 0.915437 |
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Target: 5'- gAGCGAcc--GUGGAGcucguccGGCGGCUCGUg -3' miRNA: 3'- -UCGCUuucaCACCUCc------UCGCUGAGCA- -5' |
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| 25697 | 5' | -54.3 | NC_005337.1 | + | 10399 | 0.67 | 0.896507 |
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Target: 5'- uGCucGGGcUGUGGAGGuGCGGCUgCGc -3' miRNA: 3'- uCGcuUUC-ACACCUCCuCGCUGA-GCa -5' |
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| 25697 | 5' | -54.3 | NC_005337.1 | + | 46542 | 0.67 | 0.896507 |
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Target: 5'- cAGCGAcuccauccugAAGUuc-GAGGAGCGGCUCa- -3' miRNA: 3'- -UCGCU----------UUCAcacCUCCUCGCUGAGca -5' |
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| 25697 | 5' | -54.3 | NC_005337.1 | + | 18867 | 0.67 | 0.889711 |
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Target: 5'- cGGCGAGacGGcGUccucGGAgcGGAGCGugUCGUa -3' miRNA: 3'- -UCGCUU--UCaCA----CCU--CCUCGCugAGCA- -5' |
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| 25697 | 5' | -54.3 | NC_005337.1 | + | 84586 | 0.67 | 0.889711 |
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Target: 5'- gGGCGcuGGUGUGGGugaccacGAGCGACgcCGUg -3' miRNA: 3'- -UCGCuuUCACACCUc------CUCGCUGa-GCA- -5' |
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| 25697 | 5' | -54.3 | NC_005337.1 | + | 84114 | 0.68 | 0.860208 |
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Target: 5'- gGGCGAAGcaGUcGGAGGAG-GACUUGa -3' miRNA: 3'- -UCGCUUUcaCA-CCUCCUCgCUGAGCa -5' |
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| 25697 | 5' | -54.3 | NC_005337.1 | + | 25695 | 1.07 | 0.004863 |
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Target: 5'- cAGCGAAAGUGUGGAGGAGCGACUCGUc -3' miRNA: 3'- -UCGCUUUCACACCUCCUCGCUGAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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