Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 257 | 3' | -54.9 | AC_000008.1 | + | 9427 | 0.66 | 0.632486 |
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Target: 5'- cGGCGACGacGGcGCAccgGGAGGcGGUCGa -3' miRNA: 3'- uCCGCUGC--CCaUGUca-CCUUU-CCAGC- -5' |
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| 257 | 3' | -54.9 | AC_000008.1 | + | 9776 | 0.66 | 0.62116 |
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Target: 5'- cGGCGGCGGGg-----GGAuuGGGUUGa -3' miRNA: 3'- uCCGCUGCCCaugucaCCUu-UCCAGC- -5' |
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| 257 | 3' | -54.9 | AC_000008.1 | + | 10346 | 0.67 | 0.564883 |
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Target: 5'- cGGCGGCGGcugGCGGUaGAGGGGcCa -3' miRNA: 3'- uCCGCUGCCca-UGUCAcCUUUCCaGc -5' |
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| 257 | 3' | -54.9 | AC_000008.1 | + | 23902 | 0.68 | 0.517582 |
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Target: 5'- cGGCGACGGGgACGG-GGAcgacacguccuccauGGUUGg -3' miRNA: 3'- uCCGCUGCCCaUGUCaCCUuu-------------CCAGC- -5' |
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| 257 | 3' | -54.9 | AC_000008.1 | + | 18383 | 0.77 | 0.145971 |
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Target: 5'- cGGCGGCGGGUucCAGUGGu--GGuUCGg -3' miRNA: 3'- uCCGCUGCCCAu-GUCACCuuuCC-AGC- -5' |
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| 257 | 3' | -54.9 | AC_000008.1 | + | 16707 | 1.09 | 0.000637 |
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Target: 5'- cAGGCGACGGGUACAGUGGAAAGGUCGa -3' miRNA: 3'- -UCCGCUGCCCAUGUCACCUUUCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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