Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25758 | 3' | -51.2 | NC_005337.1 | + | 74178 | 0.66 | 0.980752 |
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Target: 5'- gGAGgcGCGCUCGGGccgggagucGGcGUUCGUGUUc -3' miRNA: 3'- -CUCuuCGUGAGCUU---------CCaCGAGUACAA- -5' |
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| 25758 | 3' | -51.2 | NC_005337.1 | + | 9278 | 0.66 | 0.980752 |
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Target: 5'- -uGAGGCugUgGAuGGUGCUCcgGc- -3' miRNA: 3'- cuCUUCGugAgCUuCCACGAGuaCaa -5' |
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| 25758 | 3' | -51.2 | NC_005337.1 | + | 110417 | 0.66 | 0.978399 |
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Target: 5'- cGAGAAcGCGCggUCGAAGGU-CUCGUcGUUc -3' miRNA: 3'- -CUCUU-CGUG--AGCUUCCAcGAGUA-CAA- -5' |
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| 25758 | 3' | -51.2 | NC_005337.1 | + | 58592 | 0.66 | 0.978399 |
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Target: 5'- -cGGAGUACUUGGccGUGgUCAUGUUc -3' miRNA: 3'- cuCUUCGUGAGCUucCACgAGUACAA- -5' |
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| 25758 | 3' | -51.2 | NC_005337.1 | + | 38216 | 0.68 | 0.942244 |
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Target: 5'- uGAGuacGCACUCGAGGacGUGCUCcgGc- -3' miRNA: 3'- -CUCuu-CGUGAGCUUC--CACGAGuaCaa -5' |
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| 25758 | 3' | -51.2 | NC_005337.1 | + | 52747 | 0.68 | 0.937229 |
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Target: 5'- -uGAAGCGCgCGAAgguGGUGCUCAUc-- -3' miRNA: 3'- cuCUUCGUGaGCUU---CCACGAGUAcaa -5' |
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| 25758 | 3' | -51.2 | NC_005337.1 | + | 120773 | 0.68 | 0.936194 |
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Target: 5'- cGAGAAGUGCUCGAcucuccgguacuGGUGUUCAUc-- -3' miRNA: 3'- -CUCUUCGUGAGCUu-----------CCACGAGUAcaa -5' |
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| 25758 | 3' | -51.2 | NC_005337.1 | + | 56324 | 0.68 | 0.92639 |
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Target: 5'- cAGGAGCGCuUCGu-GGUGCUCAa--- -3' miRNA: 3'- cUCUUCGUG-AGCuuCCACGAGUacaa -5' |
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| 25758 | 3' | -51.2 | NC_005337.1 | + | 56089 | 0.74 | 0.657856 |
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Target: 5'- cGAGGAGCGCgacguggaGAAGGUGCUCGa--- -3' miRNA: 3'- -CUCUUCGUGag------CUUCCACGAGUacaa -5' |
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| 25758 | 3' | -51.2 | NC_005337.1 | + | 12166 | 1.06 | 0.008785 |
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Target: 5'- gGAGAAGCACUCGAAGGUGCUCAUGUUg -3' miRNA: 3'- -CUCUUCGUGAGCUUCCACGAGUACAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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