Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 258 | 3' | -50 | AC_000008.1 | + | 18642 | 0.66 | 0.847835 |
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Target: 5'- uCGUU--GcgGCCCGuaGCCAGUGGCAa -3' miRNA: 3'- -GCAAguCuaUGGGUgaUGGUCAUCGU- -5' |
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| 258 | 3' | -50 | AC_000008.1 | + | 14620 | 0.67 | 0.838485 |
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Target: 5'- gGUguaGGAUGCCCAcCUGuuGGUAGUc -3' miRNA: 3'- gCAag-UCUAUGGGU-GAUggUCAUCGu -5' |
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| 258 | 3' | -50 | AC_000008.1 | + | 27781 | 0.69 | 0.733245 |
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Target: 5'- uGUaCAGcccGUGCaCCGCUGCCAGUAcuGCGc -3' miRNA: 3'- gCAaGUC---UAUG-GGUGAUGGUCAU--CGU- -5' |
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| 258 | 3' | -50 | AC_000008.1 | + | 9338 | 0.69 | 0.721843 |
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Target: 5'- gCGUUCuuGUGCCCGCgccaugAgCGGUAGCc -3' miRNA: 3'- -GCAAGucUAUGGGUGa-----UgGUCAUCGu -5' |
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| 258 | 3' | -50 | AC_000008.1 | + | 4321 | 0.76 | 0.330787 |
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Target: 5'- --gUCA-AUGCCCaaGCUACCAGUGGCAg -3' miRNA: 3'- gcaAGUcUAUGGG--UGAUGGUCAUCGU- -5' |
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| 258 | 3' | -50 | AC_000008.1 | + | 17176 | 1.1 | 0.001687 |
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Target: 5'- aCGUUCAGAUACCCACUACCAGUAGCAc -3' miRNA: 3'- -GCAAGUCUAUGGGUGAUGGUCAUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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