Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25925 | 5' | -53.6 | NC_005337.1 | + | 64674 | 0.66 | 0.962478 |
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Target: 5'- gCUCGUC-GAGGGgcucagucccCUGGacgucgcgguguGCCUCUGCc -3' miRNA: 3'- aGAGCAGaCUCCU----------GACU------------UGGAGACGc -5' |
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| 25925 | 5' | -53.6 | NC_005337.1 | + | 69115 | 0.66 | 0.945701 |
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Target: 5'- cCUCGUCUGcGGGC-GAGCCgcgguccacgGCGg -3' miRNA: 3'- aGAGCAGACuCCUGaCUUGGaga-------CGC- -5' |
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| 25925 | 5' | -53.6 | NC_005337.1 | + | 86806 | 0.66 | 0.94525 |
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Target: 5'- cCUCGUCguagacgcAGGACUGAagguguccgugugcGCCUggCUGCGc -3' miRNA: 3'- aGAGCAGac------UCCUGACU--------------UGGA--GACGC- -5' |
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| 25925 | 5' | -53.6 | NC_005337.1 | + | 68269 | 0.66 | 0.942023 |
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Target: 5'- --gCGUC-GAGGACUucGACgUCUGCGg -3' miRNA: 3'- agaGCAGaCUCCUGAc-UUGgAGACGC- -5' |
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| 25925 | 5' | -53.6 | NC_005337.1 | + | 61639 | 0.67 | 0.932137 |
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Target: 5'- gCUCG-CUGAGGAagaUGGACUuguUCgugGCGg -3' miRNA: 3'- aGAGCaGACUCCUg--ACUUGG---AGa--CGC- -5' |
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| 25925 | 5' | -53.6 | NC_005337.1 | + | 84123 | 0.67 | 0.921253 |
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Target: 5'- ---aGUCggagGAGGACuUGAGCCgCUGCu -3' miRNA: 3'- agagCAGa---CUCCUG-ACUUGGaGACGc -5' |
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| 25925 | 5' | -53.6 | NC_005337.1 | + | 80901 | 0.69 | 0.867922 |
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Target: 5'- uUCUCGgugCUGAGG-UUGAGCUUCU-CGu -3' miRNA: 3'- -AGAGCa--GACUCCuGACUUGGAGAcGC- -5' |
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| 25925 | 5' | -53.6 | NC_005337.1 | + | 105081 | 1.11 | 0.003459 |
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Target: 5'- uUCUCGUCUGAGGACUGAACCUCUGCGg -3' miRNA: 3'- -AGAGCAGACUCCUGACUUGGAGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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