Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 25942 | 3' | -56.2 | NC_005337.1 | + | 108535 | 0.66 | 0.888529 |
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Target: 5'- -gACggcgCGACG-GUGCGGggcGCgCGCCu -3' miRNA: 3'- gaUGaa--GCUGCaCACGCCa--CG-GCGGu -5' |
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| 25942 | 3' | -56.2 | NC_005337.1 | + | 110721 | 0.68 | 0.782393 |
|
Target: 5'- -aGCUUgGGCGUGUacGCGGggcagGCCccGCCGg -3' miRNA: 3'- gaUGAAgCUGCACA--CGCCa----CGG--CGGU- -5' |
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| 25942 | 3' | -56.2 | NC_005337.1 | + | 118134 | 1.08 | 0.002734 |
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Target: 5'- gCUACUUCGACGUGUGCGGUGCCGCCAu -3' miRNA: 3'- -GAUGAAGCUGCACACGCCACGGCGGU- -5' |
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| 25942 | 3' | -56.2 | NC_005337.1 | + | 118761 | 0.66 | 0.887844 |
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Target: 5'- gCUGCUgcgCGACGUccuagaugacgccGgcgGCGGcGCCGUCGu -3' miRNA: 3'- -GAUGAa--GCUGCA-------------Ca--CGCCaCGGCGGU- -5' |
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| 25942 | 3' | -56.2 | NC_005337.1 | + | 126957 | 0.74 | 0.463672 |
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Target: 5'- -cGCgcgCGACGUGUGCGGguacaCGCCAc -3' miRNA: 3'- gaUGaa-GCUGCACACGCCacg--GCGGU- -5' |
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| 25942 | 3' | -56.2 | NC_005337.1 | + | 128500 | 0.67 | 0.818344 |
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Target: 5'- gUGCgucgCGuACGUGgucgcGCGGcUGCCGCCc -3' miRNA: 3'- gAUGaa--GC-UGCACa----CGCC-ACGGCGGu -5' |
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| 25942 | 3' | -56.2 | NC_005337.1 | + | 130748 | 0.66 | 0.888529 |
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Target: 5'- -cGCUgggucCGACGUGUGCaccGUGgacgcCCGCCAc -3' miRNA: 3'- gaUGAa----GCUGCACACGc--CAC-----GGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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