miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
25942 3' -56.2 NC_005337.1 + 110721 0.68 0.782393
Target:  5'- -aGCUUgGGCGUGUacGCGGggcagGCCccGCCGg -3'
miRNA:   3'- gaUGAAgCUGCACA--CGCCa----CGG--CGGU- -5'
25942 3' -56.2 NC_005337.1 + 72609 0.69 0.744263
Target:  5'- -cGCgcgGGCGUG-GUGGUGCCcGCCAc -3'
miRNA:   3'- gaUGaagCUGCACaCGCCACGG-CGGU- -5'
25942 3' -56.2 NC_005337.1 + 25020 0.7 0.647406
Target:  5'- -cGCUUCGACGUGcgcgcgGCGGacaccgaggagcugcUGCgCGCCGu -3'
miRNA:   3'- gaUGAAGCUGCACa-----CGCC---------------ACG-GCGGU- -5'
25942 3' -56.2 NC_005337.1 + 21781 0.72 0.581697
Target:  5'- --uCUUCGAcgcCGUGcUGCGG-GCCGCCu -3'
miRNA:   3'- gauGAAGCU---GCAC-ACGCCaCGGCGGu -5'
25942 3' -56.2 NC_005337.1 + 68277 0.74 0.473069
Target:  5'- -gACUUCGACGUcUGCGGccgGCCGgCGg -3'
miRNA:   3'- gaUGAAGCUGCAcACGCCa--CGGCgGU- -5'
25942 3' -56.2 NC_005337.1 + 126957 0.74 0.463672
Target:  5'- -cGCgcgCGACGUGUGCGGguacaCGCCAc -3'
miRNA:   3'- gaUGaa-GCUGCACACGCCacg--GCGGU- -5'
25942 3' -56.2 NC_005337.1 + 118134 1.08 0.002734
Target:  5'- gCUACUUCGACGUGUGCGGUGCCGCCAu -3'
miRNA:   3'- -GAUGAAGCUGCACACGCCACGGCGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.