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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25975 | 3' | -47.5 | NC_005340.1 | + | 13125 | 0.66 | 0.956669 |
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Target: 5'- gUGACUAUCgg-----CGGUGAUGCUa -3' miRNA: 3'- aGCUGAUAGaccauaaGCCAUUGCGG- -5' |
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| 25975 | 3' | -47.5 | NC_005340.1 | + | 2214 | 0.69 | 0.825249 |
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Target: 5'- gCGGCUGUCUGGaAUaCG---ACGCCg -3' miRNA: 3'- aGCUGAUAGACCaUAaGCcauUGCGG- -5' |
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| 25975 | 3' | -47.5 | NC_005340.1 | + | 855 | 0.7 | 0.815181 |
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Target: 5'- uUUGAUUAaCUGGc---CGGUGACGCUg -3' miRNA: 3'- -AGCUGAUaGACCauaaGCCAUUGCGG- -5' |
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| 25975 | 3' | -47.5 | NC_005340.1 | + | 5793 | 0.71 | 0.727579 |
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Target: 5'- aUCGACUAUgUGGUcga-GGUuuAUGCCg -3' miRNA: 3'- -AGCUGAUAgACCAuaagCCAu-UGCGG- -5' |
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| 25975 | 3' | -47.5 | NC_005340.1 | + | 5025 | 0.72 | 0.704249 |
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Target: 5'- aUCGGCg--UUGGUGUgacCGGUAcuauCGCCa -3' miRNA: 3'- -AGCUGauaGACCAUAa--GCCAUu---GCGG- -5' |
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| 25975 | 3' | -47.5 | NC_005340.1 | + | 16818 | 0.73 | 0.656697 |
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Target: 5'- gCGGCgucaGUCUGGcgg-CGGUGGCGCa -3' miRNA: 3'- aGCUGa---UAGACCauaaGCCAUUGCGg -5' |
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| 25975 | 3' | -47.5 | NC_005340.1 | + | 952 | 1.14 | 0.001723 |
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Target: 5'- cUCGACUAUCUGGUAUUCGGUAACGCCu -3' miRNA: 3'- -AGCUGAUAGACCAUAAGCCAUUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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