Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25975 | 5' | -60.5 | NC_005340.1 | + | 6396 | 0.66 | 0.299136 |
Target: 5'- uCAGCGCCGuCGGUcaggucgauagugGUCugCaGCCAGg -3' miRNA: 3'- cGUCGCGGU-GUCG-------------CAGugGcCGGUCa -5' |
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25975 | 5' | -60.5 | NC_005340.1 | + | 18880 | 0.66 | 0.284307 |
Target: 5'- aGCGGCGauaAUGGCGUUaauccccaugacaACCGGCCAc- -3' miRNA: 3'- -CGUCGCgg-UGUCGCAG-------------UGGCCGGUca -5' |
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25975 | 5' | -60.5 | NC_005340.1 | + | 6274 | 0.66 | 0.263847 |
Target: 5'- gGUGGCgcuuGCCGCAcuGCGUCugCGcgauGCCGGUg -3' miRNA: 3'- -CGUCG----CGGUGU--CGCAGugGC----CGGUCA- -5' |
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25975 | 5' | -60.5 | NC_005340.1 | + | 20332 | 0.67 | 0.250443 |
Target: 5'- cCAGCcaaGCCACAGCGUCagcACCGccccGCgCAGg -3' miRNA: 3'- cGUCG---CGGUGUCGCAG---UGGC----CG-GUCa -5' |
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25975 | 5' | -60.5 | NC_005340.1 | + | 6227 | 0.68 | 0.202378 |
Target: 5'- cCAGCGCgC-CAGCGauaUCACCGGCaucaAGa -3' miRNA: 3'- cGUCGCG-GuGUCGC---AGUGGCCGg---UCa -5' |
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25975 | 5' | -60.5 | NC_005340.1 | + | 2140 | 0.69 | 0.181487 |
Target: 5'- aGCAGCgGgCGCAGCGcUACCuaugaGGCCAGc -3' miRNA: 3'- -CGUCG-CgGUGUCGCaGUGG-----CCGGUCa -5' |
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25975 | 5' | -60.5 | NC_005340.1 | + | 18296 | 0.69 | 0.176571 |
Target: 5'- aGCAGCGCCGCc-CGUC-CCaGCCAu- -3' miRNA: 3'- -CGUCGCGGUGucGCAGuGGcCGGUca -5' |
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25975 | 5' | -60.5 | NC_005340.1 | + | 10277 | 0.69 | 0.17512 |
Target: 5'- -uGGUGCCGCcuuucugcaccucaAGCGUCGCCGucGUCAGUu -3' miRNA: 3'- cgUCGCGGUG--------------UCGCAGUGGC--CGGUCA- -5' |
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25975 | 5' | -60.5 | NC_005340.1 | + | 16366 | 0.69 | 0.167094 |
Target: 5'- cGCAGCGgCACAGCaUCAgCGcCCGGg -3' miRNA: 3'- -CGUCGCgGUGUCGcAGUgGCcGGUCa -5' |
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25975 | 5' | -60.5 | NC_005340.1 | + | 17744 | 0.72 | 0.10949 |
Target: 5'- gGCAGCGCCugcuGCGGCcauGUUACCGGCaAGc -3' miRNA: 3'- -CGUCGCGG----UGUCG---CAGUGGCCGgUCa -5' |
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25975 | 5' | -60.5 | NC_005340.1 | + | 20986 | 0.75 | 0.06494 |
Target: 5'- uCAGCGCCAgcacguuauCGGCcUCACCGGCCAu- -3' miRNA: 3'- cGUCGCGGU---------GUCGcAGUGGCCGGUca -5' |
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25975 | 5' | -60.5 | NC_005340.1 | + | 918 | 1.08 | 0.000146 |
Target: 5'- aGCAGCGCCACAGCGUCACCGGCCAGUu -3' miRNA: 3'- -CGUCGCGGUGUCGCAGUGGCCGGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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