Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25976 | 3' | -50 | NC_005340.1 | + | 17624 | 0.66 | 0.830682 |
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Target: 5'- aGCUGCGccGUCGuuuCGcUGAUGGAGGu- -3' miRNA: 3'- -CGACGUacCAGCu--GC-ACUACCUUUgu -5' |
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| 25976 | 3' | -50 | NC_005340.1 | + | 19376 | 0.67 | 0.789891 |
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Target: 5'- cCUGCAUGG-CGGCGUuGGuucUGGcgGCGg -3' miRNA: 3'- cGACGUACCaGCUGCA-CU---ACCuuUGU- -5' |
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| 25976 | 3' | -50 | NC_005340.1 | + | 25894 | 0.67 | 0.779178 |
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Target: 5'- aGCUGCAcuaaUGGcCGACGcaAUGGAuuuAGCAc -3' miRNA: 3'- -CGACGU----ACCaGCUGCacUACCU---UUGU- -5' |
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| 25976 | 3' | -50 | NC_005340.1 | + | 6112 | 0.7 | 0.640811 |
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Target: 5'- gGCUG-AUGGUCGugGUGcgcaGGAuGACAu -3' miRNA: 3'- -CGACgUACCAGCugCACua--CCU-UUGU- -5' |
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| 25976 | 3' | -50 | NC_005340.1 | + | 29961 | 0.8 | 0.178164 |
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Target: 5'- uUUGCuAUGGUCGACGUGGUGGcgguGCGu -3' miRNA: 3'- cGACG-UACCAGCUGCACUACCuu--UGU- -5' |
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| 25976 | 3' | -50 | NC_005340.1 | + | 2605 | 1.12 | 0.001065 |
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Target: 5'- cGCUGCAUGGUCGACGUGAUGGAAACAu -3' miRNA: 3'- -CGACGUACCAGCUGCACUACCUUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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