Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25977 | 3' | -57.7 | NC_005340.1 | + | 17640 | 0.66 | 0.378448 |
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Target: 5'- -cUCACGCUGGCGauuaaGCuGCGCc-GUCg -3' miRNA: 3'- aaGGUGCGACCGCg----UGcCGCGaaUAG- -5' |
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| 25977 | 3' | -57.7 | NC_005340.1 | + | 8201 | 0.66 | 0.378448 |
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Target: 5'- -cUCGCGCUGGcCGUAacCGGCGCc---- -3' miRNA: 3'- aaGGUGCGACC-GCGU--GCCGCGaauag -5' |
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| 25977 | 3' | -57.7 | NC_005340.1 | + | 8407 | 0.66 | 0.369258 |
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Target: 5'- aUCCGCGCaGGUgGCACuGCGCg---- -3' miRNA: 3'- aAGGUGCGaCCG-CGUGcCGCGaauag -5' |
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| 25977 | 3' | -57.7 | NC_005340.1 | + | 16045 | 0.67 | 0.293583 |
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Target: 5'- --aUACGCUGGCGCAgGGUcagGCUU-UCc -3' miRNA: 3'- aagGUGCGACCGCGUgCCG---CGAAuAG- -5' |
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| 25977 | 3' | -57.7 | NC_005340.1 | + | 6968 | 0.68 | 0.276293 |
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Target: 5'- -gCCAUGCcgguucugauggcaUGGCGCA-GGCGCgcAUCg -3' miRNA: 3'- aaGGUGCG--------------ACCGCGUgCCGCGaaUAG- -5' |
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| 25977 | 3' | -57.7 | NC_005340.1 | + | 17405 | 0.71 | 0.174328 |
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Target: 5'- -cCCGCGCgcaGGCGCA-GGCGCUc--- -3' miRNA: 3'- aaGGUGCGa--CCGCGUgCCGCGAauag -5' |
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| 25977 | 3' | -57.7 | NC_005340.1 | + | 19587 | 0.75 | 0.08893 |
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Target: 5'- --gCACGCUGGCGCGCa-CGUUUAUCg -3' miRNA: 3'- aagGUGCGACCGCGUGccGCGAAUAG- -5' |
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| 25977 | 3' | -57.7 | NC_005340.1 | + | 8614 | 0.77 | 0.058279 |
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Target: 5'- -aCCgGCGCUGGCGCagGCGGCGCgggUGUg -3' miRNA: 3'- aaGG-UGCGACCGCG--UGCCGCGa--AUAg -5' |
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| 25977 | 3' | -57.7 | NC_005340.1 | + | 2906 | 1.06 | 0.00034 |
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Target: 5'- cUUCCACGCUGGCGCACGGCGCUUAUCc -3' miRNA: 3'- -AAGGUGCGACCGCGUGCCGCGAAUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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