Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25978 | 3' | -51.8 | NC_005340.1 | + | 6293 | 0.66 | 0.744865 |
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Target: 5'- cGUcUGCGCGAUgccGG-UGAaucUGUCGACc -3' miRNA: 3'- uCAaACGCGCUGa--CCaACU---GCAGCUG- -5' |
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| 25978 | 3' | -51.8 | NC_005340.1 | + | 116 | 0.66 | 0.73366 |
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Target: 5'- uGGUUgUGCGCGugUGGc--GCGUCugaGGCg -3' miRNA: 3'- -UCAA-ACGCGCugACCaacUGCAG---CUG- -5' |
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| 25978 | 3' | -51.8 | NC_005340.1 | + | 3828 | 0.67 | 0.686655 |
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Target: 5'- uGGUcugGCGCauccugaGACUGGaaacUGGCGUUGACc -3' miRNA: 3'- -UCAaa-CGCG-------CUGACCa---ACUGCAGCUG- -5' |
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| 25978 | 3' | -51.8 | NC_005340.1 | + | 16615 | 0.69 | 0.582337 |
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Target: 5'- --aUUGCGCGGC-GGUgcuguuccaGACgGUCGACg -3' miRNA: 3'- ucaAACGCGCUGaCCAa--------CUG-CAGCUG- -5' |
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| 25978 | 3' | -51.8 | NC_005340.1 | + | 17256 | 0.7 | 0.524837 |
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Target: 5'- gGGgcUGCGCGACcugaacGGUcaGGCGUCGAa -3' miRNA: 3'- -UCaaACGCGCUGa-----CCAa-CUGCAGCUg -5' |
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| 25978 | 3' | -51.8 | NC_005340.1 | + | 3132 | 1.09 | 0.001059 |
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Target: 5'- cAGUUUGCGCGACUGGUUGACGUCGACc -3' miRNA: 3'- -UCAAACGCGCUGACCAACUGCAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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