Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25978 | 5' | -50.9 | NC_005340.1 | + | 9256 | 0.66 | 0.7774 |
Target: 5'- cUGUCAcCGGCGGCGcgucggcaaaugGCGGCugacauuGCAAAa -3' miRNA: 3'- aACAGUaGCCGUUGU------------UGCCG-------CGUUUc -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 2519 | 0.66 | 0.767484 |
Target: 5'- --aUgGUCGGCGA--ACGGgGCGAAGu -3' miRNA: 3'- aacAgUAGCCGUUguUGCCgCGUUUC- -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 3922 | 0.66 | 0.760797 |
Target: 5'- -cG-CAUCGGCGucgagggugauaccuGCGACGgGCGCAu-- -3' miRNA: 3'- aaCaGUAGCCGU---------------UGUUGC-CGCGUuuc -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 9188 | 0.66 | 0.744981 |
Target: 5'- -cGUCAcuggcUCaGGCAGCggUGGCuCAGGGg -3' miRNA: 3'- aaCAGU-----AG-CCGUUGuuGCCGcGUUUC- -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 28186 | 0.67 | 0.71024 |
Target: 5'- cUG-UAUCaGCAugGGCGGCGCGc-- -3' miRNA: 3'- aACaGUAGcCGUugUUGCCGCGUuuc -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 17715 | 0.67 | 0.686595 |
Target: 5'- -cGggGUCGGUAuuGCGACGGCGgGAAc -3' miRNA: 3'- aaCagUAGCCGU--UGUUGCCGCgUUUc -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 16055 | 0.67 | 0.686595 |
Target: 5'- --uUUAUCGGUGAUAcGCuGGCGCAGGGu -3' miRNA: 3'- aacAGUAGCCGUUGU-UG-CCGCGUUUC- -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 24980 | 0.68 | 0.650696 |
Target: 5'- gUUGUCAuuaUCGGCAAUGGCGcugcauGCGCGu-- -3' miRNA: 3'- -AACAGU---AGCCGUUGUUGC------CGCGUuuc -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 28371 | 0.68 | 0.650696 |
Target: 5'- -cGUCAgCGGCaucAugGACGGCgGCAAAc -3' miRNA: 3'- aaCAGUaGCCG---UugUUGCCG-CGUUUc -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 17570 | 0.68 | 0.63867 |
Target: 5'- ---cCAUCaGCGaaACGACGGCGCAGc- -3' miRNA: 3'- aacaGUAGcCGU--UGUUGCCGCGUUuc -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 6138 | 0.68 | 0.63867 |
Target: 5'- ---aCAcCGGCGACccACGGCGCAuAGa -3' miRNA: 3'- aacaGUaGCCGUUGu-UGCCGCGUuUC- -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 10313 | 0.71 | 0.452927 |
Target: 5'- -gGUCAcggCGGCGuACAACGGgGCGGGa -3' miRNA: 3'- aaCAGUa--GCCGU-UGUUGCCgCGUUUc -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 27698 | 0.72 | 0.381054 |
Target: 5'- -gGUCAUCaGCucGCAGCGGCGUAu-- -3' miRNA: 3'- aaCAGUAGcCGu-UGUUGCCGCGUuuc -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 21843 | 0.73 | 0.343546 |
Target: 5'- -aGcCAUUGGCGACAAagUGGCgGCAGAGg -3' miRNA: 3'- aaCaGUAGCCGUUGUU--GCCG-CGUUUC- -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 16822 | 0.74 | 0.300506 |
Target: 5'- -cGUCAgucUGGCGGCggUGGCGCAGu- -3' miRNA: 3'- aaCAGUa--GCCGUUGuuGCCGCGUUuc -5' |
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25978 | 5' | -50.9 | NC_005340.1 | + | 3090 | 1.06 | 0.001727 |
Target: 5'- aUUGUCAUCGGCAACAACGGCGCAAAGc -3' miRNA: 3'- -AACAGUAGCCGUUGUUGCCGCGUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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