Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25980 | 3' | -52.2 | NC_005340.1 | + | 17331 | 0.66 | 0.619181 |
Target: 5'- ----uGGUCAGGCGCuugacaaGGCaAAGCGCGAa -3' miRNA: 3'- cuuuuCCGGUUCGUG-------CCG-UUCGUGUU- -5' |
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25980 | 3' | -52.2 | NC_005340.1 | + | 10567 | 0.67 | 0.584179 |
Target: 5'- --uGAGcGCCGGGUuuuGCGGCAugucaaucAGCGCAGa -3' miRNA: 3'- cuuUUC-CGGUUCG---UGCCGU--------UCGUGUU- -5' |
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25980 | 3' | -52.2 | NC_005340.1 | + | 9163 | 0.68 | 0.524926 |
Target: 5'- ----cGGUCGAGCuguacguuaACGGCGAGCugGu -3' miRNA: 3'- cuuuuCCGGUUCG---------UGCCGUUCGugUu -5' |
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25980 | 3' | -52.2 | NC_005340.1 | + | 11108 | 0.68 | 0.490472 |
Target: 5'- uGAGGGGCUGAGCGUGGCGGGguCu- -3' miRNA: 3'- cUUUUCCGGUUCGUGCCGUUCguGuu -5' |
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25980 | 3' | -52.2 | NC_005340.1 | + | 28286 | 0.69 | 0.457107 |
Target: 5'- --cGAGGCCGcGCACGGUcuGAGCGu-- -3' miRNA: 3'- cuuUUCCGGUuCGUGCCG--UUCGUguu -5' |
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25980 | 3' | -52.2 | NC_005340.1 | + | 6260 | 0.71 | 0.346373 |
Target: 5'- --uGAGGCCGuauugcAGCGCGuaGGGCGCAAu -3' miRNA: 3'- cuuUUCCGGU------UCGUGCcgUUCGUGUU- -5' |
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25980 | 3' | -52.2 | NC_005340.1 | + | 1261 | 0.73 | 0.263548 |
Target: 5'- -uGAGGuGCC-AGCAUGaGCAAGCGCAAg -3' miRNA: 3'- cuUUUC-CGGuUCGUGC-CGUUCGUGUU- -5' |
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25980 | 3' | -52.2 | NC_005340.1 | + | 8303 | 1.07 | 0.00093 |
Target: 5'- aGAAAAGGCCAAGCACGGCAAGCACAAg -3' miRNA: 3'- -CUUUUCCGGUUCGUGCCGUUCGUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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