Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25982 | 3' | -58.4 | NC_005340.1 | + | 17831 | 0.66 | 0.391983 |
Target: 5'- cGCCAgaaaugcaggcgcUgcGCaaacaGGCGCGCCaGCuCGGUGAc -3' miRNA: 3'- -CGGU-------------AaaCGg----CUGCGCGG-CG-GCCACU- -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 17685 | 0.66 | 0.383747 |
Target: 5'- uGCCGguaacaugGCCGcagcAgGCGCUGCCGGg-- -3' miRNA: 3'- -CGGUaaa-----CGGC----UgCGCGGCGGCCacu -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 9385 | 0.66 | 0.374731 |
Target: 5'- uGCCGUcgggUGCCugcugucgccuGAUGCGCUGCUGcUGAc -3' miRNA: 3'- -CGGUAa---ACGG-----------CUGCGCGGCGGCcACU- -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 20410 | 0.66 | 0.374731 |
Target: 5'- gGUCAgg-GUaucaGGCGCGCC-CCGGUGc -3' miRNA: 3'- -CGGUaaaCGg---CUGCGCGGcGGCCACu -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 20722 | 0.66 | 0.36586 |
Target: 5'- uGCCA-UUGCCGAC-CGCgGagcuuauaCCGGUGu -3' miRNA: 3'- -CGGUaAACGGCUGcGCGgC--------GGCCACu -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 8559 | 0.66 | 0.36586 |
Target: 5'- cCCGc--GCCGcCuGCGCCagcGCCGGUGAu -3' miRNA: 3'- cGGUaaaCGGCuG-CGCGG---CGGCCACU- -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 9357 | 0.66 | 0.36586 |
Target: 5'- cCCGUaUGCCGcccgaaaGCGCCaGUCGGUGc -3' miRNA: 3'- cGGUAaACGGCug-----CGCGG-CGGCCACu -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 2816 | 0.67 | 0.348557 |
Target: 5'- -aCAUcaGUCacaGCGCGCuCGCCGGUGGg -3' miRNA: 3'- cgGUAaaCGGc--UGCGCG-GCGGCCACU- -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 11750 | 0.67 | 0.340126 |
Target: 5'- uGCCGUg-GCUGGCGUgggcguuuucuGUCGaCCGGUGGg -3' miRNA: 3'- -CGGUAaaCGGCUGCG-----------CGGC-GGCCACU- -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 14345 | 0.67 | 0.339292 |
Target: 5'- gGCCAUccucugaUUGaaGAUGCGCCGCacCGGcGAa -3' miRNA: 3'- -CGGUA-------AACggCUGCGCGGCG--GCCaCU- -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 6279 | 0.69 | 0.264017 |
Target: 5'- cGCU---UGCCGcacugcgucuGCGCGauGCCGGUGAa -3' miRNA: 3'- -CGGuaaACGGC----------UGCGCggCGGCCACU- -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 5674 | 0.69 | 0.244047 |
Target: 5'- gGCaggUUGCCGAUGCGCUuCUGGcUGAu -3' miRNA: 3'- -CGguaAACGGCUGCGCGGcGGCC-ACU- -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 12147 | 0.7 | 0.225349 |
Target: 5'- cGCCAUc-GCagGACaCGCCGCCGGUa- -3' miRNA: 3'- -CGGUAaaCGg-CUGcGCGGCGGCCAcu -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 6714 | 0.7 | 0.207881 |
Target: 5'- cCCGcUUUGaCCGGCGUGCCGcCCGGcucggGAg -3' miRNA: 3'- cGGU-AAAC-GGCUGCGCGGC-GGCCa----CU- -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 11154 | 0.73 | 0.141129 |
Target: 5'- gGCCGUagUGCUGACGgGCgCGUCGGcGAu -3' miRNA: 3'- -CGGUAa-ACGGCUGCgCG-GCGGCCaCU- -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 6078 | 0.74 | 0.119023 |
Target: 5'- uGCCGUUcuaugcGCCGugG-GUCGCCGGUGu -3' miRNA: 3'- -CGGUAAa-----CGGCugCgCGGCGGCCACu -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 2492 | 0.84 | 0.018425 |
Target: 5'- uGCCGgaugUGUCGuacuCGCGCCGCCGGUGGu -3' miRNA: 3'- -CGGUaa--ACGGCu---GCGCGGCGGCCACU- -5' |
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25982 | 3' | -58.4 | NC_005340.1 | + | 9315 | 1.1 | 0.000187 |
Target: 5'- cGCCAUUUGCCGACGCGCCGCCGGUGAc -3' miRNA: 3'- -CGGUAAACGGCUGCGCGGCGGCCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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