Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25983 | 3' | -58.7 | NC_005340.1 | + | 27960 | 0.66 | 0.364419 |
Target: 5'- uGUGCCaAUGCGgcguUCCGUCgugauGAGGCUcGGCa -3' miRNA: 3'- gUACGG-UGCGU----AGGCGG-----CUCCGA-CUG- -5' |
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25983 | 3' | -58.7 | NC_005340.1 | + | 1597 | 0.66 | 0.363532 |
Target: 5'- --cGCgGCGCAaugggauUCCucGCCGAcaaGGCUGAUg -3' miRNA: 3'- guaCGgUGCGU-------AGG--CGGCU---CCGACUG- -5' |
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25983 | 3' | -58.7 | NC_005340.1 | + | 9817 | 0.66 | 0.346105 |
Target: 5'- uGUGCCGCGUacaaagcggcgggAUCCagacugcaugGCUGAacuGGCUGACc -3' miRNA: 3'- gUACGGUGCG-------------UAGG----------CGGCU---CCGACUG- -5' |
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25983 | 3' | -58.7 | NC_005340.1 | + | 24814 | 0.66 | 0.330111 |
Target: 5'- -cUGCCAUGCGUaCCGgucaaCGA-GCUGGCa -3' miRNA: 3'- guACGGUGCGUA-GGCg----GCUcCGACUG- -5' |
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25983 | 3' | -58.7 | NC_005340.1 | + | 4531 | 0.66 | 0.321914 |
Target: 5'- uCAUGCaggCGCGCAUCguCGCUGAccuguuuacGGCUGAa -3' miRNA: 3'- -GUACG---GUGCGUAG--GCGGCU---------CCGACUg -5' |
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25983 | 3' | -58.7 | NC_005340.1 | + | 1107 | 0.66 | 0.321914 |
Target: 5'- aAUGgUACGag-CCGCCGgucagcuucuccGGGCUGGCa -3' miRNA: 3'- gUACgGUGCguaGGCGGC------------UCCGACUG- -5' |
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25983 | 3' | -58.7 | NC_005340.1 | + | 21585 | 0.67 | 0.309118 |
Target: 5'- -cUGCCGC-CAauuugCCGCCaucgcuaauaaaaaaGGGGCUGGCa -3' miRNA: 3'- guACGGUGcGUa----GGCGG---------------CUCCGACUG- -5' |
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25983 | 3' | -58.7 | NC_005340.1 | + | 5970 | 0.67 | 0.283225 |
Target: 5'- --cGCCAUGCAaCCGCCuauGGCaGAUg -3' miRNA: 3'- guaCGGUGCGUaGGCGGcu-CCGaCUG- -5' |
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25983 | 3' | -58.7 | NC_005340.1 | + | 28245 | 0.69 | 0.231602 |
Target: 5'- uGUGCCAgCGCG-CCGCCcaugcugauacagcgGGGGCUGuCu -3' miRNA: 3'- gUACGGU-GCGUaGGCGG---------------CUCCGACuG- -5' |
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25983 | 3' | -58.7 | NC_005340.1 | + | 9261 | 0.69 | 0.217031 |
Target: 5'- aCcgGCgGCGCGUCgGCaaauGGcGGCUGACa -3' miRNA: 3'- -GuaCGgUGCGUAGgCGg---CU-CCGACUG- -5' |
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25983 | 3' | -58.7 | NC_005340.1 | + | 15266 | 0.74 | 0.09221 |
Target: 5'- --aGCCGCGUAUUCuugggguGCCGGGGCUGGa -3' miRNA: 3'- guaCGGUGCGUAGG-------CGGCUCCGACUg -5' |
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25983 | 3' | -58.7 | NC_005340.1 | + | 9309 | 0.77 | 0.055842 |
Target: 5'- -uUGCCgACGCG-CCGCCGgugacAGGCUGGCa -3' miRNA: 3'- guACGG-UGCGUaGGCGGC-----UCCGACUG- -5' |
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25983 | 3' | -58.7 | NC_005340.1 | + | 10056 | 1.1 | 0.000155 |
Target: 5'- aCAUGCCACGCAUCCGCCGAGGCUGACg -3' miRNA: 3'- -GUACGGUGCGUAGGCGGCUCCGACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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