Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25983 | 5' | -50.9 | NC_005340.1 | + | 6645 | 0.66 | 0.780888 |
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Target: 5'- -uGAAcgcUCCCCgagccgGGCGGCACGccggucAAAGCg -3' miRNA: 3'- cuCUUuu-AGGGG------CCGUCGUGC------UUUCG- -5' |
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| 25983 | 5' | -50.9 | NC_005340.1 | + | 30001 | 0.67 | 0.690189 |
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Target: 5'- -------aCCCCGcCGGCACuGAAAGCg -3' miRNA: 3'- cucuuuuaGGGGCcGUCGUG-CUUUCG- -5' |
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| 25983 | 5' | -50.9 | NC_005340.1 | + | 28102 | 0.68 | 0.678407 |
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Target: 5'- ------uUCUCCGGgucgauagccuCAGCAUGAAAGCg -3' miRNA: 3'- cucuuuuAGGGGCC-----------GUCGUGCUUUCG- -5' |
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| 25983 | 5' | -50.9 | NC_005340.1 | + | 17749 | 0.69 | 0.618992 |
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Target: 5'- -------aCCCCGGCAGCGCcugcuGCg -3' miRNA: 3'- cucuuuuaGGGGCCGUCGUGcuuu-CG- -5' |
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| 25983 | 5' | -50.9 | NC_005340.1 | + | 9252 | 0.7 | 0.536655 |
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Target: 5'- cAGccuGUCaCCGGCGGCGCGucGGCa -3' miRNA: 3'- cUCuuuUAGgGGCCGUCGUGCuuUCG- -5' |
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| 25983 | 5' | -50.9 | NC_005340.1 | + | 28171 | 0.7 | 0.529747 |
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Target: 5'- aAGAGAcagCCCCcgcuguaucagcaugGGCGGCGCGcuGGCa -3' miRNA: 3'- cUCUUUua-GGGG---------------CCGUCGUGCuuUCG- -5' |
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| 25983 | 5' | -50.9 | NC_005340.1 | + | 2426 | 0.71 | 0.480206 |
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Target: 5'- -cGAAA--CCaCCGGCGGCGCGAGuacgacacauccGGCa -3' miRNA: 3'- cuCUUUuaGG-GGCCGUCGUGCUU------------UCG- -5' |
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| 25983 | 5' | -50.9 | NC_005340.1 | + | 5354 | 0.71 | 0.480206 |
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Target: 5'- -uGAAg--CCCCGGC-GCGCGugauGAGCa -3' miRNA: 3'- cuCUUuuaGGGGCCGuCGUGCu---UUCG- -5' |
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| 25983 | 5' | -50.9 | NC_005340.1 | + | 10012 | 1.13 | 0.000641 |
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Target: 5'- gGAGAAAAUCCCCGGCAGCACGAAAGCa -3' miRNA: 3'- -CUCUUUUAGGGGCCGUCGUGCUUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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