Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25984 | 5' | -56.1 | NC_005340.1 | + | 13860 | 0.66 | 0.502351 |
Target: 5'- uGGCuGUacaGAUGCCacccCUGAUCGUCg -3' miRNA: 3'- gCCGuCAgaaCUACGGc---GACUGGCAG- -5' |
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25984 | 5' | -56.1 | NC_005340.1 | + | 28319 | 0.66 | 0.491568 |
Target: 5'- -cGCcGUCcaUGAUGCCGCUGacGCCGg- -3' miRNA: 3'- gcCGuCAGa-ACUACGGCGAC--UGGCag -5' |
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25984 | 5' | -56.1 | NC_005340.1 | + | 25878 | 0.68 | 0.390516 |
Target: 5'- uCGGCAG-CUUcGGUGCCGCUcauACCcUCu -3' miRNA: 3'- -GCCGUCaGAA-CUACGGCGAc--UGGcAG- -5' |
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25984 | 5' | -56.1 | NC_005340.1 | + | 19353 | 0.68 | 0.371986 |
Target: 5'- gCGGCGGUCUggucGAUGuuGcCUGACUcuuuGUUg -3' miRNA: 3'- -GCCGUCAGAa---CUACggC-GACUGG----CAG- -5' |
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25984 | 5' | -56.1 | NC_005340.1 | + | 8673 | 0.7 | 0.266624 |
Target: 5'- -cGCcGUCUUGAggGCgCGCUGACCGcCu -3' miRNA: 3'- gcCGuCAGAACUa-CG-GCGACUGGCaG- -5' |
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25984 | 5' | -56.1 | NC_005340.1 | + | 6161 | 0.71 | 0.226756 |
Target: 5'- uGGC-GUCUUGAUGCCGgUGAUa-UCg -3' miRNA: 3'- gCCGuCAGAACUACGGCgACUGgcAG- -5' |
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25984 | 5' | -56.1 | NC_005340.1 | + | 16378 | 0.72 | 0.208784 |
Target: 5'- uGGCGGUCgaGGUGgucaUGCgUGGCCGUCa -3' miRNA: 3'- gCCGUCAGaaCUACg---GCG-ACUGGCAG- -5' |
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25984 | 5' | -56.1 | NC_005340.1 | + | 10134 | 1.1 | 0.000295 |
Target: 5'- uCGGCAGUCUUGAUGCCGCUGACCGUCa -3' miRNA: 3'- -GCCGUCAGAACUACGGCGACUGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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