Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25985 | 3' | -53.9 | NC_005340.1 | + | 10189 | 0.66 | 0.629455 |
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Target: 5'- cGCUUUuaccaguaccaCCGGCacgguGGCGGUAaugGACUCCGa -3' miRNA: 3'- -UGAGA-----------GGUCGgu---CCGCUAU---UUGAGGC- -5' |
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| 25985 | 3' | -53.9 | NC_005340.1 | + | 423 | 0.66 | 0.617799 |
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Target: 5'- cGCUCaccgcgugccguUCCAGCUuauGGGCGGcAAGC-CCGa -3' miRNA: 3'- -UGAG------------AGGUCGG---UCCGCUaUUUGaGGC- -5' |
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| 25985 | 3' | -53.9 | NC_005340.1 | + | 6762 | 0.66 | 0.606158 |
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Target: 5'- aAC-CUCaucaGGUUGGGCGGUGGACgCCGg -3' miRNA: 3'- -UGaGAGg---UCGGUCCGCUAUUUGaGGC- -5' |
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| 25985 | 3' | -53.9 | NC_005340.1 | + | 6649 | 0.67 | 0.525933 |
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Target: 5'- cGCUCcCCgAGCCGGGCGGc--ACgCCGg -3' miRNA: 3'- -UGAGaGG-UCGGUCCGCUauuUGaGGC- -5' |
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| 25985 | 3' | -53.9 | NC_005340.1 | + | 8895 | 0.68 | 0.503694 |
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Target: 5'- aGCUCUCCAGUCGGGaauccGUAuGC-CCGu -3' miRNA: 3'- -UGAGAGGUCGGUCCgc---UAUuUGaGGC- -5' |
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| 25985 | 3' | -53.9 | NC_005340.1 | + | 13218 | 1.08 | 0.000722 |
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Target: 5'- aACUCUCCAGCCAGGCGAUAAACUCCGc -3' miRNA: 3'- -UGAGAGGUCGGUCCGCUAUUUGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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