Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25986 | 5' | -53.6 | NC_005340.1 | + | 16929 | 0.66 | 0.637279 |
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Target: 5'- -uGCCCGAuauGUuGUCGCugGCCGCUAa -3' miRNA: 3'- agUGGGCUuacCAuUAGCG--UGGCGGU- -5' |
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| 25986 | 5' | -53.6 | NC_005340.1 | + | 1615 | 0.66 | 0.602434 |
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Target: 5'- cUCGCCgacaaGGcUGaUGGUCGCACCGCUg -3' miRNA: 3'- -AGUGGg----CUuACcAUUAGCGUGGCGGu -5' |
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| 25986 | 5' | -53.6 | NC_005340.1 | + | 5108 | 0.68 | 0.511463 |
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Target: 5'- gUCGCCCGA--GGUGucaGUCGUGCUGgCGa -3' miRNA: 3'- -AGUGGGCUuaCCAU---UAGCGUGGCgGU- -5' |
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| 25986 | 5' | -53.6 | NC_005340.1 | + | 2491 | 0.71 | 0.368862 |
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Target: 5'- ---gCCGGAUGugucGUAcUCGCGCCGCCGg -3' miRNA: 3'- agugGGCUUAC----CAUuAGCGUGGCGGU- -5' |
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| 25986 | 5' | -53.6 | NC_005340.1 | + | 20420 | 0.72 | 0.292701 |
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Target: 5'- gCGCCCGGAUGGUcaggguaucAggCGCGCC-CCGg -3' miRNA: 3'- aGUGGGCUUACCA---------UuaGCGUGGcGGU- -5' |
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| 25986 | 5' | -53.6 | NC_005340.1 | + | 26907 | 0.74 | 0.222501 |
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Target: 5'- -aGCCUGAAUGcGUGcguuuugauguuuAUCGCACCGCUg -3' miRNA: 3'- agUGGGCUUAC-CAU-------------UAGCGUGGCGGu -5' |
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| 25986 | 5' | -53.6 | NC_005340.1 | + | 19462 | 0.75 | 0.20174 |
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Target: 5'- cCugCCGggUGGUGuugCGCcggggcaucagcuugACCGCCAg -3' miRNA: 3'- aGugGGCuuACCAUua-GCG---------------UGGCGGU- -5' |
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| 25986 | 5' | -53.6 | NC_005340.1 | + | 13372 | 1.1 | 0.000623 |
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Target: 5'- gUCACCCGAAUGGUAAUCGCACCGCCAg -3' miRNA: 3'- -AGUGGGCUUACCAUUAGCGUGGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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