Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 25988 | 3' | -53.6 | NC_005340.1 | + | 2201 | 0.66 | 0.668125 |
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Target: 5'- aUACGA---CGCCGGggcaaccgaCGUCACGCa -3' miRNA: 3'- -GUGCUaaaGCGGCCaca------GCAGUGCGa -5' |
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| 25988 | 3' | -53.6 | NC_005340.1 | + | 2500 | 0.66 | 0.645096 |
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Target: 5'- gGCGAcagUGCCGGaugUGUCGuacUCGCGCc -3' miRNA: 3'- gUGCUaaaGCGGCC---ACAGC---AGUGCGa -5' |
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| 25988 | 3' | -53.6 | NC_005340.1 | + | 20289 | 0.67 | 0.610484 |
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Target: 5'- -uCGAcUUUGCCgucGGUGUCGUCGaGCUc -3' miRNA: 3'- guGCUaAAGCGG---CCACAGCAGUgCGA- -5' |
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| 25988 | 3' | -53.6 | NC_005340.1 | + | 1724 | 0.69 | 0.445067 |
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Target: 5'- aCACG----CGUCGGUGuUCGUCugGCg -3' miRNA: 3'- -GUGCuaaaGCGGCCAC-AGCAGugCGa -5' |
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| 25988 | 3' | -53.6 | NC_005340.1 | + | 7572 | 0.74 | 0.248873 |
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Target: 5'- gGCGGUUuugucucaauggUCGCCGGUGUUGUuCugGUg -3' miRNA: 3'- gUGCUAA------------AGCGGCCACAGCA-GugCGa -5' |
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| 25988 | 3' | -53.6 | NC_005340.1 | + | 10671 | 0.75 | 0.223086 |
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Target: 5'- aUAC-AUUUCGCCaGUGUCGcCGCGCUc -3' miRNA: 3'- -GUGcUAAAGCGGcCACAGCaGUGCGA- -5' |
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| 25988 | 3' | -53.6 | NC_005340.1 | + | 16410 | 1.08 | 0.000862 |
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Target: 5'- cCACGAUUUCGCCGGUGUCGUCACGCUg -3' miRNA: 3'- -GUGCUAAAGCGGCCACAGCAGUGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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