Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
25989 | 3' | -62.1 | NC_005340.1 | + | 28907 | 0.66 | 0.273603 |
Target: 5'- aGCGCCAUCGCa-UCAGGCUaaaGCCa -3' miRNA: 3'- -CGCGGUGGCGgcGGUCUGAcugCGGc -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 12623 | 0.66 | 0.272914 |
Target: 5'- uCGCCGaacaugaaaaaucCCGUCGUCAucCUGAUGCCa -3' miRNA: 3'- cGCGGU-------------GGCGGCGGUcuGACUGCGGc -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 8245 | 0.66 | 0.266781 |
Target: 5'- cCGCCAgCCGUgCGCCAGACagUGACcaGCUu -3' miRNA: 3'- cGCGGU-GGCG-GCGGUCUG--ACUG--CGGc -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 1635 | 0.66 | 0.260098 |
Target: 5'- uCG-CACCGCUGUCAaGCUGuACGCUGu -3' miRNA: 3'- cGCgGUGGCGGCGGUcUGAC-UGCGGC- -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 1332 | 0.66 | 0.260098 |
Target: 5'- cGCGCUggcauGCUGCauCCAgugugaccGACUGGCGCCa -3' miRNA: 3'- -CGCGG-----UGGCGgcGGU--------CUGACUGCGGc -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 5968 | 0.66 | 0.260098 |
Target: 5'- uGCGCCAUgcaaCCGCCuauGGCaGAUGCUGg -3' miRNA: 3'- -CGCGGUGgc--GGCGGu--CUGaCUGCGGC- -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 18835 | 0.66 | 0.253551 |
Target: 5'- aCGCCAuuaUCGCCGCU--GCUGGCGUg- -3' miRNA: 3'- cGCGGU---GGCGGCGGucUGACUGCGgc -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 17492 | 0.66 | 0.247141 |
Target: 5'- aGCGCCAgCGCU-CCGaGCUGAUGCa- -3' miRNA: 3'- -CGCGGUgGCGGcGGUcUGACUGCGgc -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 27642 | 0.67 | 0.228713 |
Target: 5'- uGCGauaCGCCGCUGCgAG-CUGAUGaCCc -3' miRNA: 3'- -CGCg--GUGGCGGCGgUCuGACUGC-GGc -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 447 | 0.67 | 0.222834 |
Target: 5'- -aGCCAgUGCCGCC-GACcugaugGACGCUc -3' miRNA: 3'- cgCGGUgGCGGCGGuCUGa-----CUGCGGc -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 27844 | 0.67 | 0.211461 |
Target: 5'- uGCGUguCCGa---CGGGCUGGCGCCGu -3' miRNA: 3'- -CGCGguGGCggcgGUCUGACUGCGGC- -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 27484 | 0.67 | 0.211461 |
Target: 5'- cGCGCCAUgcaGCCGCaaugcgacgCAGACgaccACGCCa -3' miRNA: 3'- -CGCGGUGg--CGGCG---------GUCUGac--UGCGGc -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 17861 | 0.67 | 0.209799 |
Target: 5'- cGCGCCagcucggugacaauACCGCCGCUucuGCUGAUgaugcaGCCa -3' miRNA: 3'- -CGCGG--------------UGGCGGCGGuc-UGACUG------CGGc -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 22239 | 0.67 | 0.205964 |
Target: 5'- -aGCUACCGgCGCCAGAUggagUGAgGCa- -3' miRNA: 3'- cgCGGUGGCgGCGGUCUG----ACUgCGgc -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 6960 | 0.67 | 0.205964 |
Target: 5'- aCGaCUACCGgcugCGCCAGACUGcCGCgGg -3' miRNA: 3'- cGC-GGUGGCg---GCGGUCUGACuGCGgC- -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 17456 | 0.67 | 0.200591 |
Target: 5'- aGCGCCugCGCCugcgcgcggGUCGGACUgGugGUg- -3' miRNA: 3'- -CGCGGugGCGG---------CGGUCUGA-CugCGgc -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 16613 | 0.68 | 0.180304 |
Target: 5'- uGCGCgGCggUGCUGuuCCAGACggucGACGCCGu -3' miRNA: 3'- -CGCGgUG--GCGGC--GGUCUGa---CUGCGGC- -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 9377 | 0.69 | 0.162297 |
Target: 5'- gGUGCCuGCUGUCGCCugaugcgcugcugcuGACUGGCGCg- -3' miRNA: 3'- -CGCGG-UGGCGGCGGu--------------CUGACUGCGgc -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 8570 | 0.69 | 0.157519 |
Target: 5'- uGCGCCAgCGCCGgu-GAUUGugGUCa -3' miRNA: 3'- -CGCGGUgGCGGCgguCUGACugCGGc -5' |
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25989 | 3' | -62.1 | NC_005340.1 | + | 11660 | 0.69 | 0.153286 |
Target: 5'- uUGCCGCCGCaagggcaugUGCCgAGAUUGAaagaaCGCCGg -3' miRNA: 3'- cGCGGUGGCG---------GCGG-UCUGACU-----GCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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